高表达 CD14+外周血单核细胞的 ALX/FPR2 和 BLT1 在类风湿关节炎低疾病活动度和缓解状态下的表现：一项初步研究。

High CD14+ peripheral monocytes expression of ALX/FPR2 and BLT1 in low disease activity and remission status in rheumatoid arthritis: a piltot study.

机构信息

Rheumatology Unit, Department of Emergence Medicine and Transplantation (DETO), University of Bari "Aldo Moro", Bari, and Division of Rheumatology, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.

Rheumatology Unit, Department of Emergence Medicine and Transplantation (DETO), University of Bari "Aldo Moro", Bari, Italy.

出版信息

Clin Exp Rheumatol. 2022 May;40(5):1019-1024. doi: 10.55563/clinexprheumatol/akl60y. Epub 2021 Sep 7.

DOI:10.55563/clinexprheumatol/akl60y

PMID:34494955

Abstract

OBJECTIVES

Specialised pro-resolving mediator (SPM) can dampen the acute inflammation through ERV1, ALX/FPR2 and BLT1 cell receptors and it is conceivable that their expression is dysregulated during chronic inflammation. The aim of this study was to evaluate the expression of ERV1, ALX/FPR2 and BLT1 on peripheral blood (PB) cells from rheumatoid arthritis (RA) patients.

METHODS

At baseline, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), clinimetric indexes (28-joint disease activity score (DAS28) and clinical disease activity index (CDAI)), and PB samples were collected from 33 RA patients. Based on DAS28, patients were divided into high-moderate (H-Mo/RA, DAS28≥3.2) and low-remission (L-Rem/RA, DAS28<3.2) disease activity group. Cell membrane expression of ERV1, ALX/FPR2 and BLT1 on CD3pos, CD19pos, CD14pos cells and granulocytes was assessed by multi-parametric flow-cytometry analysis. Nine healthy controls (HC) were also studied.

RESULTS

Sixteen H-Mo/RA and 17 L-Rem/RA patients were identified. The percentage of BLT1posCD14pos cells was significantly higher in L-Rem/RA (47.17%) than in H-Mo/RA (14.27%) group (p=0.005). Likewise, the percentage ALX/FPR2pos CD14pos cells was significantly higher in L-Rem/RA (33.02%) than in H-Mo/RA (8.77%; p=0.04) patients. An inverse correlation between BLT1posCD14pos cell percentage and DAS28 (r=-0.42; p=0.01), CDAI (r=-0.51; p=0.003), ESR (r=-0.39; p=0.025) and CRP (r=-0.40; p=0.02), ALX/FPR2posCD14pos cell percentage and CRP (r=-0.39; p=0.02) were found, while SPM-receptors mean fluorescence intensity (MFI) was not different between HC and L-Rem/RA patients.

CONCLUSIONS

ALX/FPR2 and BLT1 receptors expression mirrors RA disease activity arising as potential biomarkers of inflammatory regulation.

摘要

目的

特殊的促解决介质 (SPM) 可以通过 ERV1、ALX/FPR2 和 BLT1 细胞受体来抑制急性炎症，并且可以想象，它们的表达在慢性炎症期间会失调。本研究的目的是评估类风湿关节炎 (RA) 患者外周血 (PB) 细胞中 ERV1、ALX/FPR2 和 BLT1 的表达。

方法

在基线时，采集了 33 名 RA 患者的红细胞沉降率 (ESR)、C 反应蛋白 (CRP)、临床指标（28 关节疾病活动评分 (DAS28) 和临床疾病活动指数 (CDAI)）和 PB 样本。根据 DAS28，患者被分为高-中度 (H-Mo/RA，DAS28≥3.2) 和低缓解 (L-Rem/RA，DAS28<3.2) 疾病活动组。通过多参数流式细胞术分析评估 CD3pos、CD19pos、CD14pos 细胞和粒细胞上 ERV1、ALX/FPR2 和 BLT1 的细胞膜表达。还研究了 9 名健康对照 (HC)。

结果

确定了 16 名 H-Mo/RA 和 17 名 L-Rem/RA 患者。L-Rem/RA（47.17%）组中 BLT1posCD14pos 细胞的百分比明显高于 H-Mo/RA（14.27%）组（p=0.005）。同样，L-Rem/RA（33.02%）患者中 ALX/FPR2posCD14pos 细胞的百分比也明显高于 H-Mo/RA（8.77%；p=0.04）患者。BLT1posCD14pos 细胞百分比与 DAS28（r=-0.42；p=0.01）、CDAI（r=-0.51；p=0.003）、ESR（r=-0.39；p=0.025）和 CRP（r=-0.40；p=0.02）呈负相关，而 ALX/FPR2posCD14pos 细胞百分比与 CRP（r=-0.39；p=0.02）呈负相关，而 SPM 受体平均荧光强度 (MFI) 在 HC 和 L-Rem/RA 患者之间没有差异。