Universidade Federal de Santa Catarina - UFSC, Department of Dentistry, Florianópolis, SC, Brazil.
AC Camargo Cancer Center, Department of Head & Neck, São Paulo, SP, Brazil.
Braz Oral Res. 2021 Sep 3;35:e075. doi: 10.1590/1807-3107bor-2021.vol35.0075. eCollection 2021.
Smoking has been shown to alter innate and adaptive immune responses and is directly associated with the onset of oral squamous cell carcinoma (OSCC). The purpose of this study was to evaluate the effect of cigarette smoke exposure on dendritic cells (DCs) from OSCC patients. CD1a and CD83 antibodies were used to identify immature and mature DCs, respectively, by immunohistochemistry in OSCC samples of 24 smokers and 24 non-smokers. Density of DCs was calculated in intra and peritumoral areas. Clinical and microscopic findings were reviewed and analyzed for all patients. Smokers with OSCC had a lower density of intra and peritumoral DCs when compared to non-smokers. Tumors classified as moderately/poorly differentiated had lower peritumoral CD1a+ DCs than well-differentiated tumors (p < 0.001). Smoking contributed to a depletion of immature and mature DCs in the OSCC.
吸烟已被证明会改变先天和适应性免疫反应，并与口腔鳞状细胞癌（OSCC）的发生直接相关。本研究旨在评估香烟烟雾暴露对来自 OSCC 患者的树突状细胞（DC）的影响。通过免疫组织化学方法，使用 CD1a 和 CD83 抗体分别鉴定 OSCC 样本中的未成熟和成熟 DC。在肿瘤内和肿瘤周围区域计算 DC 的密度。对所有患者进行临床和显微镜检查结果的回顾和分析。与不吸烟者相比，患有 OSCC 的吸烟者的肿瘤内和肿瘤周围 DC 密度较低。中/低分化肿瘤的肿瘤周围 CD1a+ DC 密度低于高分化肿瘤（p<0.001）。吸烟导致 OSCC 中未成熟和成熟 DC 的耗竭。
