Abels Kristen, Salvo-Halloran Elizabeth M, White Dawn, Ali Monsur, Agarwal Nisha R, Leung Vincent, Ali Muntakim, Sidawi Mariam, Capretta Alfredo, Brennan John D, Nease Jake, Filipe Carlos D M
Department of Chemical Engineering, McMaster University, 1280 Main St. West, Hamilton, Ontario L8S 4M1, Canada.
Biointerfaces Institute, McMaster University, 1280 Main St. West, Hamilton, Ontario L8S 4M1, Canada.
ACS Omega. 2021 Aug 17;6(34):22439-22446. doi: 10.1021/acsomega.1c03460. eCollection 2021 Aug 31.
Colorimetric assays typically offer a rapid and convenient method to assess analytes that span healthcare monitoring to water quality testing. However, such tests can only provide qualitative results when employed in resource-limited settings or require bulky and expensive equipment such as lab spectrophotometers to allow quantitative measurements. In this paper, we report on the use of a handheld colorimeter to quantitatively determine the concentration of analytes in a manner that is independent of ambient lighting or initial sample color. The method combines the response of the sensor with first-principles modeling that better describes the nature of the assay compared to linear-in-parameters regression modeling that is typically performed in other studies. This method was successfully demonstrated using a number of colorimetric assays: (1) determination of solution pH using a universal indicator, (2) quantification of the DNase presence using a DNA-gold nanoparticle assay, and (3) quantification of the concentration of the antibiotic tetracycline using a cell-based assay.
比色测定法通常提供了一种快速便捷的方法来评估各种分析物，涵盖从医疗保健监测到水质检测等领域。然而，当在资源有限的环境中使用时，此类测试只能提供定性结果，或者需要诸如实验室分光光度计等笨重且昂贵的设备才能进行定量测量。在本文中，我们报告了使用手持式比色计以一种独立于环境照明或初始样品颜色的方式定量测定分析物浓度的方法。该方法将传感器的响应与第一性原理建模相结合，与其他研究中通常进行的参数线性回归建模相比，能更好地描述测定的本质。使用多种比色测定法成功证明了该方法：（1）使用通用指示剂测定溶液pH值；（2）使用DNA - 金纳米颗粒测定法对脱氧核糖核酸酶（DNase）的存在进行定量；（3）使用基于细胞的测定法对抗生素四环素的浓度进行定量。
