Gee Clifford T, Kehoe Eric, Pomerantz William C K, Penn R Lee
Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, United States.
Janesville Waldorf Pemberton High School, Janesville, Minnesota 56048, United States.
J Chem Educ. 2017 Jul 10;94(7):941-945. doi: 10.1021/acs.jchemed.6b00676. Epub 2017 May 3.
Proteins are involved in nearly every biological process, which makes them of interest to a range of scientists. Previous work has shown that hand-held cameras can be used to determine the concentration of colored analytes in solution, and this paper extends the approach to reactions involving a color change in order to quantify protein concentration (e.g., green to blue). Herein, we describe the successful use of smartphone colorimetry to quantify protein concentration using two common colorimetric biochemical methods, the Bradford and biuret assays. The ease of the experimental setup makes these lab experiments accessible to a wide range of students and can be used as both high school and college level laboratory experiments.
蛋白质几乎参与了每一个生物过程，这使得它们成为众多科学家感兴趣的对象。先前的研究表明，手持相机可用于测定溶液中有色分析物的浓度，本文将该方法扩展到涉及颜色变化的反应中，以定量蛋白质浓度（例如，从绿色变为蓝色）。在此，我们描述了使用智能手机比色法通过两种常见的比色生化方法（考马斯亮蓝法和双缩脲法）来定量蛋白质浓度的成功应用。实验设置简便，使得这些实验室实验可供广大学生使用，并且既可以用作高中水平的实验室实验，也可以用作大学水平的实验室实验。
