Endoscopy Center, The First People's Hospital of Wenling, Zhejiang, China.
Department of Gastroenterology, The First People's Hospital of Wenling, Zhejiang, China.
Nutr Cancer. 2022;74(5):1872-1881. doi: 10.1080/01635581.2021.1972125. Epub 2021 Sep 9.
The progression of esophagus cancer (EC) is associated with the alterative expressions of multiple microRNAs (miRs). MiR-1-3p is reported to inhibit the development of EC by targeting TAGLN2. Quercetin (Que) is a natural compound capable of antagonizing esophagus carcinoma (EC). In the current study, the role of miR-1-3p/TAGLN2 axis in the anti-EC function of Que was explored. Human EC cell lines KYSE-510 and TE-7 were treated with Que. Then the effects of Que on the growth and metastasis of EC cells, and on the activity of miR-1-3p/TAGLN2 axis were detected. The interaction between Que and miR-1-3p axis was further assessed by inhibiting miR-1-3p level in EC cells. The results showed that the treatment of Que impaired the growth and induced cell apoptosis in EC cells. The invasive ability of EC cells was also suppressed by Que. At molecular level, the expression of miR-1-3p was induced, while the expression of TAGLN2 was suppressed by Que. Moreover, the anti-EC effects of Que were blocked by miR-1-3p inhibition, which was represented by the restored growth and invasion of EC cells. Collectively, the current study demonstrated that Que exerted inhibitory effects on EC cells by inducing miR-1-3p.Supplemental data for this article is available online at https://doi.org/10.1080/01635581.2021.1972125.
食管癌（EC）的进展与多种 microRNAs（miRs）的改变表达有关。有报道称，miR-1-3p 通过靶向 TAGLN2 抑制 EC 的发展。槲皮素（Que）是一种能够拮抗食管癌（EC）的天然化合物。在本研究中，探讨了 miR-1-3p/TAGLN2 轴在 Que 抗 EC 功能中的作用。用 Que 处理人 EC 细胞系 KYSE-510 和 TE-7。然后检测 Que 对 EC 细胞生长和转移以及 miR-1-3p/TAGLN2 轴活性的影响。通过抑制 EC 细胞中 miR-1-3p 的水平进一步评估了 Que 与 miR-1-3p 轴之间的相互作用。结果表明，Que 处理可损害 EC 细胞的生长并诱导细胞凋亡。Que 还抑制了 EC 细胞的侵袭能力。在分子水平上，Que 诱导了 miR-1-3p 的表达，同时抑制了 TAGLN2 的表达。此外，miR-1-3p 抑制阻断了 Que 的抗 EC 作用，这表现为 EC 细胞的生长和侵袭恢复。总之，本研究表明 Que 通过诱导 miR-1-3p 对 EC 细胞发挥抑制作用。本文的补充数据可在 https://doi.org/10.1080/01635581.2021.1972125 在线获取。
