Nakasone N, Iwanaga M, Eeckels R
Okinawa Department of Bacteriology, University of the Ryukyus School of Medicine, Okinawa, Japan.
Trans R Soc Trop Med Hyg. 1987;81(5):876-8. doi: 10.1016/0035-9203(87)90059-9.
91 strains of Vibrio cholerae O1, isolated in Bangladesh in January 1986, were examined for their biological behaviour and sensitivity to 6 antimicrobial agents. Biotyping indicated that 60 of the isolates belonged to the classical biotype and 31 to the El Tor biotype. 21 El Tor strains revealed beta-haemolysis on blood agar plates, but only 8 showed complete haemolysis in broth. Serotyping indicated 79 Ogawa, 10 Inaba, and 2 Hikojima. Phage typing showed that all classical vibrios belonged to Mukerjee's phage type 1. El Tor vibrios were classified into 4 groups: one strain each in type 1 and type 5, 19 in type 4, and 10 in an untypable group. Prophage typing of El Tor vibrios identified 14 strains of Ubol type, 16 of cured Celebes type, and one of original Celebes type. No strain was resistant to tetracycline, minocycline, chloramphenicol, streptomycin, amoxicillin or nalidixic acid. The classical vibrios differed from those isolated before 1973 in toxin production pattern.
1986年1月在孟加拉国分离出的91株霍乱弧菌O1菌株，对其生物学特性及对6种抗菌药物的敏感性进行了检测。生物分型表明，60株分离株属于古典生物型，31株属于埃尔托生物型。21株埃尔托菌株在血琼脂平板上呈现β溶血，但在肉汤中仅有8株完全溶血。血清分型显示79株小川型、10株稻叶型和2株彦岛型。噬菌体分型表明，所有古典弧菌均属于穆克吉噬菌体1型。埃尔托弧菌分为4组：1型和5型各1株，4型19株，1株无法分型。埃尔托弧菌的前噬菌体分型鉴定出14株乌博尔型、16株治愈的西里伯斯型和1株原始西里伯斯型。没有菌株对四环素、米诺环素、氯霉素、链霉素、阿莫西林或萘啶酸耐药。古典弧菌在毒素产生模式上与1973年以前分离的菌株不同。
