Department of Mechanical Engineering, Rochester Institute of Technology, Rochester, NY 14623, United States; Department of Microsystems Engineering, Rochester Institute of Technology, Rochester, NY 14623, United States.
Department of Mechanical Engineering, Rochester Institute of Technology, Rochester, NY 14623, United States.
J Colloid Interface Sci. 2022 Jan 15;606(Pt 2):1609-1616. doi: 10.1016/j.jcis.2021.08.119. Epub 2021 Aug 21.
Efficient separation of blood cells and plasma is key for numerous molecular diagnosis and therapeutics applications. Despite various microfluidics-based separation strategies having been developed, there is still a need for a simple, reliable, and multiplexing separation device that can process a large volume of blood. Here we show a microbead-packed deformable microfluidic system that can efficiently separate highly purified plasma from whole blood, as well as retrieve blocked blood cells from the device. To support and rationalize the experimental validation of the proposed device, a highly accurate model is constructed to help understand the link between the mechanical properties of the microfluidics, flow rate, and microbeads packing/leaking based on the microscope imaging and the optical coherence tomography (OCT) scanning. This deformable nano-sieve device is expected to offer a new solution for centrifuge-free diagnosis and treatment of bloodborne diseases and contribute to the design of next-generation deformable microfluidics for separation applications.
高效分离血液细胞和血浆是许多分子诊断和治疗应用的关键。尽管已经开发出了各种基于微流控的分离策略，但仍然需要一种简单、可靠且可进行大量血液处理的多路复用分离装置。在这里，我们展示了一种微珠填充的可变形微流控系统，该系统可以高效地从全血中分离出高度纯净的血浆，并且还可以从设备中回收堵塞的血细胞。为了支持和验证所提出设备的实验验证，我们构建了一个高度精确的模型，以帮助理解微流控的机械性能、流速以及基于显微镜成像和光学相干断层扫描（OCT）扫描的微珠填充/泄漏之间的联系。这种可变形纳米筛装置有望为无离心诊断和治疗血液传播疾病提供新的解决方案，并为下一代用于分离应用的可变形微流控设计做出贡献。
