D D Pant Interdisciplinary Research Laboratory, University of Allahabad, Prayagraj, 211002, India.
Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Allahabad, 211002, India.
Plant Physiol Biochem. 2021 Oct;167:713-722. doi: 10.1016/j.plaphy.2021.08.011. Epub 2021 Aug 9.
The present study was aimed to investigate copper (Cu) toxicity alleviatory potential of silicon in Vigna radiata L. (mung bean) seedlings. Moreover, attention has also been paid to find out whether endogenous nitric oxide (NO) has any role in Si-governed alleviation of Cu stress. The length of root and shoot, fresh weight, and biochemical attributes were adversely affected by Cu exposure. However, application of Si rescued negative effects of Cu. Cu exposure decreased cell viability, and enhanced cell death and levels of oxidative stress markers (O‾, HO and MDA), but Si significantly mitigated these effects of Cu. Application of Cu substantially stimulated the activities of superoxide dismutase and guaiacol peroxidase while inhibited activity of catalase. However, Si addition reversed this effect of Cu. Ascorbate and glutathione contents in roots and shoots were declined by Cu but stimulated by Si. Moreover, we noticed that addition of Nω-nitro--arginine methyl ester hydrochloride (L-NAME) and sodium tungstate (Tung) further augmented Cu toxicity but addition of sodium nitroprusside rescued adverse effects of L-NAME and Tung. Altogether, data suggest that though Si was able in alleviating Cu toxicity in mung bean seedlings but it requires endogenous nitric oxide.
本研究旨在探究硅对绿豆（Vigna radiata L.）幼苗铜毒性的缓解作用。此外，我们还关注了内源性一氧化氮（NO）在硅调控缓解铜胁迫中的作用。结果表明，铜暴露会抑制绿豆幼苗的根长、苗长、鲜重以及一些生化指标，而硅的添加则缓解了铜对绿豆幼苗的毒害作用。铜暴露会降低细胞活力，增加细胞死亡和氧化应激标志物（O‾、HO 和 MDA）的水平，而硅则能显著减轻铜的这些效应。铜暴露会显著刺激超氧化物歧化酶和愈创木酚过氧化物酶的活性，同时抑制过氧化氢酶的活性，但硅的添加则逆转了铜的这种作用。铜暴露会降低根和苗中的抗坏血酸和谷胱甘肽含量，但硅的添加则会刺激其含量增加。此外，我们还注意到，添加 L-NAME 和 Tung 会进一步增强铜的毒性，但添加 SNP 则能挽救 L-NAME 和 Tung 的毒性。综上所述，数据表明，硅虽然能够缓解绿豆幼苗的铜毒性，但需要内源性一氧化氮的参与。
