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手性整体硅胶高效液相色谱柱用于对映体分离和测定：手性选择剂的功能化和选择剂-选择物相互作用的识别。

Chiral Monolithic Silica-Based HPLC Columns for Enantiomeric Separation and Determination: Functionalization of Chiral Selector and Recognition of Selector-Selectand Interaction.

机构信息

College of Pharmacy, King Khalid University, Abha 62529, Saudi Arabia.

Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA.

出版信息

Molecules. 2021 Aug 29;26(17):5241. doi: 10.3390/molecules26175241.

DOI:10.3390/molecules26175241
PMID:34500675
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8434329/
Abstract

This review draws attention to the use of chiral monolithic silica HPLC columns for the enantiomeric separation and determination of chiral compounds. Properties and advantages of monolithic silica HPLC columns are also highlighted in comparison to conventional particle-packed, fused-core, and sub-2-µm HPLC columns. Nano-LC capillary monolithic silica columns as well as polymeric-based and hybrid-based monolithic columns are also demonstrated to show good enantioresolution abilities. Methods for introducing the chiral selector into the monolithic silica column in the form of mobile phase additive, by encapsulation and surface coating, or by covalent functionalization are described. The application of molecular modeling methods to elucidate the selector-selectand interaction is discussed. An application for enantiomeric impurity determination is also considered.

摘要

本文综述了手性整体硅胶 HPLC 柱在对映异构体分离和手性化合物测定中的应用。与传统的颗粒填充、熔融核和亚 2μm HPLC 柱相比，还强调了整体硅胶 HPLC 柱的特性和优势。还展示了纳米 LC 毛细管整体硅胶柱以及基于聚合物和混合基质的整体硅胶柱具有良好的对映体分辨率能力。介绍了将手性选择剂以流动相添加剂、包封和表面涂层或共价功能化的形式引入整体硅胶柱中的方法。讨论了应用分子建模方法阐明选择剂-选择物相互作用的情况。还考虑了用于对映异构体杂质测定的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd22/8434329/574b93092019/molecules-26-05241-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd22/8434329/d3b01de06a94/molecules-26-05241-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd22/8434329/97145d6467cb/molecules-26-05241-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd22/8434329/dc11f3a3d02c/molecules-26-05241-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd22/8434329/c9a46866bb27/molecules-26-05241-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd22/8434329/574b93092019/molecules-26-05241-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd22/8434329/d3b01de06a94/molecules-26-05241-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd22/8434329/97145d6467cb/molecules-26-05241-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd22/8434329/dc11f3a3d02c/molecules-26-05241-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd22/8434329/c9a46866bb27/molecules-26-05241-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd22/8434329/574b93092019/molecules-26-05241-g005.jpg

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