Chirality Program, Faculty of Science and Technology, University of Canberra, Canberra, ACT 2601, Australia.
Pharmaceutical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt.
Molecules. 2019 Feb 26;24(5):833. doi: 10.3390/molecules24050833.
A new functionalized polymer monolithic capillary with a macrocyclic antibiotic, namely colistin sulfate, as chiral selector was prepared via the copolymerization of binary monomer mixtures consisting of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA) in porogenic solvents namely 1-propanol and 1,4-butanediol, in the presence of azobisiso-butyronitrile (AIBN) as initiator and colistin sulfate. The prepared capillaries were investigated for the enantioselective nano-LC separation of a group of racemic pharmaceuticals, namely, α- and β-blockers, anti-inflammatory drugs, antifungal drugs, norepinephrine-dopamine reuptake inhibitors, catecholamines, sedative hypnotics, antihistaminics, anticancer drugs, and antiarrhythmic drugs. Acceptable separation was achieved for many drugs using reversed phase chromatographic conditions with no separation achieved under normal phase conditions. Colistin sulfate appears to be useful addition to the available macrocyclic antibiotic chiral phases used in liquid chromatography.
一种新型的功能化聚合物整体毛细管，以硫酸粘菌素作为手性选择剂，通过在致孔溶剂 1-丙醇和 1,4-丁二醇中聚合由甲基丙烯酸缩水甘油酯（GMA）和乙二醇二甲基丙烯酸酯（EGDMA）组成的二元单体混合物，并在偶氮二异丁腈（AIBN）作为引发剂和硫酸粘菌素的存在下制备而成。研究了制备的毛细管对一组外消旋药物的手性纳流液相色谱分离性能，这些药物包括α-和β-受体阻滞剂、消炎药、抗真菌药、去甲肾上腺素-多巴胺再摄取抑制剂、儿茶酚胺、镇静催眠药、抗组胺药、抗癌药和抗心律失常药。使用反相色谱条件可以实现许多药物的可接受分离，但在正相条件下无法实现分离。硫酸粘菌素似乎是对液相色谱中可用的大环抗生素手性相的有用补充。
