Graduate Institute of Acupuncture Science, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan.
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan.
Molecules. 2021 Sep 2;26(17):5338. doi: 10.3390/molecules26175338.
Ergosta-7,9(11),22-trien-3β-ol (EK100) was isolated from the Taiwan-specific medicinal fungus Antrodia camphorata, which is known for its health-promotion and anti-aging effects in folk medicine. Alzheimer's disease (AD) is a major aging-associated disease. We investigated the efficacy and potential mechanism of ergosta-7,9(11),22-trien-3β-ol for AD symptoms. with the pan-neuronal overexpression of human amyloid-β (Aβ) was used as the AD model. We compared the life span, motor function, learning, memory, oxidative stress, and biomarkers of microglia activation and inflammation of the ergosta-7,9(11),22-trien-3β-ol-treated group to those of the untreated control. Ergosta-7,9(11),22-trien-3β-ol treatment effectively improved the life span, motor function, learning, and memory of the AD model compared to the untreated control. Biomarkers of microglia activation and inflammation were reduced, while the ubiquitous lipid peroxidation, catalase activity, and superoxide dismutase activity remained unchanged. In conclusion, ergosta-7,9(11),22-trien-3β-ol rescues AD deficits by modulating microglia activation but not oxidative stress.
麦角甾-7,9(11),22-三烯-3β-醇（EK100）是从台湾特有的药用真菌樟芝中分离出来的，樟芝在民间医学中以其促进健康和抗衰老的功效而闻名。阿尔茨海默病（AD）是一种与衰老相关的主要疾病。我们研究了麦角甾-7,9(11),22-三烯-3β-醇对 AD 症状的疗效和潜在机制。我们使用泛神经元过表达人淀粉样蛋白-β（Aβ）作为 AD 模型。我们将麦角甾-7,9(11),22-三烯-3β-醇处理组与未处理对照组的寿命、运动功能、学习、记忆、氧化应激以及小胶质细胞激活和炎症的生物标志物进行了比较。与未处理对照组相比，麦角甾-7,9(11),22-三烯-3β-醇处理有效地提高了 AD 模型的寿命、运动功能、学习和记忆。小胶质细胞激活和炎症的生物标志物减少，而普遍的脂质过氧化、过氧化氢酶活性和超氧化物歧化酶活性保持不变。总之，麦角甾-7,9(11),22-三烯-3β-醇通过调节小胶质细胞激活而不是氧化应激来挽救 AD 缺陷。
