Department of Physiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara 252-0374, Kanagawa, Japan.
Department of Nephrology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Kumamoto, Japan.
Molecules. 2021 Sep 5;26(17):5399. doi: 10.3390/molecules26175399.
The kidney is a main site of erythropoietin production in the body. We developed a new method for the detection of Epo protein by deglycosylation-coupled Western blotting. Detection of deglycosylated Epo enables the examination of small changes in Epo production. Using this method, we investigated the effects of angiotensin II (ATII) on Epo production in the kidney. ATII stimulated the plasma Epo concentration; Epo, HIF2α, and PHD2 mRNA expression in nephron segments in the renal cortex and outer medulla; and Epo protein expression in the renal cortex. In situ hybridization and immunohistochemistry revealed that ATII stimulates Epo mRNA and protein expression not only in proximal tubules but also in collecting ducts, especially in intercalated cells. These data support the regulation of Epo production in the kidney by the renin-angiotensin-aldosterone system (RAS).
肾脏是体内促红细胞生成素产生的主要部位。我们开发了一种新的方法，通过去糖基化结合 Western blot 检测 Epo 蛋白。检测去糖基化的 Epo 可以检测到 Epo 产生的微小变化。使用这种方法，我们研究了血管紧张素 II (ATII) 对肾脏中 Epo 产生的影响。ATII 刺激血浆 Epo 浓度；Epo、HIF2α 和 PHD2 mRNA 在肾皮质和外髓质的肾单位段中的表达；以及 Epo 蛋白在肾皮质中的表达。原位杂交和免疫组织化学显示，ATII 不仅刺激近端小管中 Epo mRNA 和蛋白的表达，也刺激集合管中 Epo mRNA 和蛋白的表达，尤其是在闰细胞中。这些数据支持肾素-血管紧张素-醛固酮系统 (RAS) 对肾脏中 Epo 产生的调节。
