Tulska Ewa, Aniszewska Monika, Gendek Arkadiusz
Department of Biosystems Engineering, Institute of Mechanical Engineering, Warsaw University of Life Sciences, Nowoursynowska 164, 02-787 Warsaw, Poland.
Materials (Basel). 2021 Aug 29;14(17):4913. doi: 10.3390/ma14174913.
The objective of the study was to elucidate the kinematics of cone opening in the European larch ( Mill.) during a four-step seed extraction process and to determine optimum process time on that basis. Each step lasted 8 h with 10 min of water immersion between the steps. The study also described the microscopic cellular structure of scales in cones with a moisture content of 5% and 20%, as well as evaluated changes in cell wall thickness. The obtained results were compared with the structural investigations of scales conducted using scanning electron microscopy (SEM) of characteristic sites on the inner and outer sides of the scales. The greatest increment in the scale opening angle was noted on the first day of the process (34°) and in scales from the middle cone segment (39°). In scales with a moisture content of 5% and 20%, the greatest changes in cell wall thickness were recorded for large cells (57%). The inner and outer structure of scales differed in terms of the presence and size of cells depending on the moisture content of the cones (5%, 10%, or 20%). The study demonstrated that the moisture content of cones was the crucial determinant of the cellular structure and opening of scales in larch cones. The scale opening angle increased with decreasing moisture content but did not differ significantly for various segments of cones or various hours of the consecutive days of the process. This finding may lead to reducing the seed extraction time for larch cones. The internal and external structure of scales differed depending on moisture content, which also determined the size and wall thickness of cells.
该研究的目的是阐明欧洲落叶松球果在四步种子提取过程中的球果张开运动学,并在此基础上确定最佳处理时间。每一步持续8小时,步骤之间有10分钟的水浸处理。该研究还描述了含水量为5%和20%的球果鳞片的微观细胞结构,并评估了细胞壁厚度的变化。将所得结果与使用扫描电子显微镜(SEM)对鳞片内侧和外侧特征部位进行的鳞片结构研究进行比较。在处理的第一天,鳞片张开角度的最大增量为34°,在球果中间部分的鳞片中为39°。在含水量为5%和20%的鳞片中,大型细胞的细胞壁厚度变化最大(57%)。根据球果的含水量(5%、10%或20%),鳞片的内部和外部结构在细胞的存在和大小方面存在差异。该研究表明,球果的含水量是落叶松球果细胞结构和鳞片张开的关键决定因素。鳞片张开角度随含水量降低而增加,但在球果的不同部分或处理连续几天的不同时间之间没有显著差异。这一发现可能会缩短落叶松球果的种子提取时间。鳞片的内部和外部结构因含水量而异,含水量也决定了细胞的大小和壁厚。