Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padua, Viale dell'Università 16, 35020 Legnaro, Italy.
Department of Comparative Biomedicine and Food Science (BCA), University of Padua, Viale dell'Università 16, 35020 Legnaro, Italy.
Int J Mol Sci. 2021 Sep 2;22(17):9527. doi: 10.3390/ijms22179527.
Maize root responds to nitrate by modulating its development through the coordinated action of many interacting players. Nitric oxide is produced in primary root early after the nitrate provision, thus inducing root elongation. In this study, RNA sequencing was applied to discover the main molecular signatures distinguishing the response of maize root to nitrate according to their dependency on, or independency of, nitric oxide, thus discriminating the signaling pathways regulated by nitrate through nitric oxide from those regulated by nitrate itself of by further downstream factors. A set of subsequent detailed functional annotation tools (Gene Ontology enrichment, MapMan, KEGG reconstruction pathway, transcription factors detection) were used to gain further information and the lateral root density was measured both in the presence of nitrate and in the presence of nitrate plus cPTIO, a specific NO scavenger, and compared to that observed for N-depleted roots. Our results led us to identify six clusters of transcripts according to their responsiveness to nitric oxide and to their regulation by nitrate provision. In general, shared and specific features for the six clusters were identified, allowing us to determine the overall root response to nitrate according to its dependency on nitric oxide.
玉米根通过许多相互作用的因子的协调作用来调节其发育，从而对硝酸盐做出反应。在硝酸盐供应后早期，一氧化氮就会在主根中产生，从而诱导根伸长。在这项研究中，我们应用 RNA 测序来发现主要的分子特征，根据它们对一氧化氮的依赖或独立性，来区分玉米根对硝酸盐的反应，从而区分受一氧化氮调节的信号通路与受硝酸盐本身或进一步下游因子调节的信号通路。一组后续的详细功能注释工具（GO 富集分析、MapMan、KEGG 重建途径、转录因子检测）被用来获取更多的信息，并在存在硝酸盐和存在硝酸盐加 cPTIO（一种特定的 NO 清除剂）的情况下测量侧根密度，并与氮饥饿根观察到的密度进行比较。我们的结果导致我们根据对一氧化氮的反应性和硝酸盐供应的调节，将转录物分为六个簇。总的来说，我们确定了六个簇的共享和特定特征，从而能够根据一氧化氮的依赖性来确定根对硝酸盐的整体反应。
