Department of Microbiology and Parasitology, Institute of Biology, Jan Kochanowski University in Kielce, Kielce, Poland.
Świętokrzyskie State Provincial Sanitary Inspection, Kielce, Poland.
Sci Rep. 2021 Sep 9;11(1):17886. doi: 10.1038/s41598-021-96848-w.
Rheumatoid arthritis (RA) is one of the most common autoimmune diseases worldwide. Due to high heterogeneity in disease manifestation, accurate and fast diagnosis of RA is difficult. This study analyzed the potential relationship between the infrared (IR) spectra obtained by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and the presence of autoantibodies and antibodies against urease in sera. Additionally, the wave number of the IR spectrum that enabled the best differentiation between patients and healthy blood donors was investigated. Using a mathematical model involving principal component analysis and discriminant analysis, it was shown that the presence of anti-citrullinated protein antibody, rheumatoid factor, anti-neutrophil cytoplasmic antibodies, and anti-nuclear antibodies correlated significantly with the wave numbers in the IR spectra of the tested sera. The most interesting findings derived from determination of the best predictors for distinguishing RA. Characteristic features included an increased reaction with urease mimicking peptides and a correspondence with particular nucleic acid bands. Taken together, the results demonstrated the potential application of ATR-FTIR in the study of RA and identified potential novel markers of the disease.
类风湿关节炎（RA）是全球最常见的自身免疫性疾病之一。由于疾病表现存在高度异质性，因此准确、快速地诊断 RA 较为困难。本研究分析了衰减全反射傅里叶变换红外光谱（ATR-FTIR）获得的红外光谱与血清中自身抗体和尿素酶抗体存在之间的潜在关系。此外，还研究了能使患者与健康献血者血清的红外光谱最佳区分的波数。通过运用包含主成分分析和判别分析的数学模型，研究表明抗瓜氨酸化蛋白抗体、类风湿因子、抗中性粒细胞胞浆抗体和抗核抗体的存在与检测血清的红外光谱波数显著相关。最有趣的发现来自于确定区分 RA 的最佳预测因子的研究。特征包括对模拟肽的尿素酶反应增加以及与特定核酸带的对应关系。总的来说，研究结果表明 ATR-FTIR 在 RA 研究中具有潜在的应用价值，并确定了疾病的潜在新型标志物。
