Fluorescence Spectroscopy Research Laboratory, Department of Chemistry, Shivaji University, Kolhapur, Maharashtra, 416004, India.
Department of Chemistry, Lal Bahadur Shastri College of Arts, Science and Commerce, Satara, Maharashtra, 415002, India.
Sci Rep. 2021 Sep 9;11(1):17996. doi: 10.1038/s41598-021-97552-5.
Herein, for the first time the carbon dots (CDs) were synthesized by reflux method from sawmill waste material. We also represent a novel strategy based on fluorescent CDs for determination of ponceau 4R and allura red dyes in soft drinks. Interestingly, both the dyes were sensitive and showed effective fluorescence quenching of the CDs owing to the interaction between them. The analytical applicability of CDs were evaluated for detection of both the dyes with a good linear relationship between the concentration range of 0.0 to 3.0 µg mL and having detection limit 0.45 and 0.47 µg mL for allura red and ponceau 4R dyes respectively. Meanwhile, the potential application of this novel fluorescent probe for dyes determination in real samples was validated in different soft drink samples with good accuracy and precision. Thus, these findings provides new insights for the potential risk assessment of both the dyes. Moreover, CDs acted as an excellent fluorescent material in cellular imaging owing to their cellular uptake and localization.
在此，我们首次通过回流法从锯木厂废料中合成了碳点（CDs）。我们还提出了一种基于荧光 CDs 的新策略，用于测定软饮料中的食用红色 4R 号和诱惑红染料。有趣的是，由于它们之间的相互作用，两种染料都具有敏感性，并对 CDs 表现出有效的荧光猝灭。用 CDs 评估了分析适用性，用于检测两种染料，其浓度范围为 0.0 至 3.0 µg mL，检测限分别为 0.45 和 0.47 µg mL 用于诱惑红和食用红色 4R 染料。同时，该新型荧光探针在不同软饮料样品中的染料测定中的潜在应用具有良好的准确性和精密度，得到了验证。因此，这些发现为这两种染料的潜在风险评估提供了新的见解。此外，由于 CDs 具有细胞摄取和定位能力，因此其可作为细胞成像的优异荧光材料。
