QTL Map of Early- and Late-Stage Perennial Regrowth in .

Numerous climate change threats will necessitate a shift toward more sustainable agricultural practices during the 21st century. Conversion of annual crops to perennials that are capable of regrowing over multiple yearly growth cycles could help to facilitate this transition. Perennials can capture greater amounts of carbon and access more water and soil nutrients compared to annuals. In principle it should be possible to identify genes that confer perenniality from wild relatives and transfer them into existing breeding lines to create novel perennial crops. Two major loci controlling perennial regrowth in the maize relative were previously mapped to chromosome 2 ( and chromosome 7 (). Here we extend this work by mapping perennial regrowth in segregating populations involving and the maize inbreds P39 and Hp301 using QTL-seq and traditional QTL mapping approaches. The results confirmed the existence of a major perennial regrowth QTL on chromosome 2 (). Although we did not observe the QTL in these populations, we discovered a third QTL on chromosome 8 which we named . The locus exerts its strongest effect late in the regrowth cycle. Neither nor overlapped with tiller number QTL scored in the same population, suggesting specific roles in the perennial phenotype. Our data, along with prior work, indicate that perennial regrowth in maize is conferred by relatively few major QTL.