Laboratory of Genetics, University of Wisconsin-Madison, Wisconsin 53706.
National Maize Improvement Center, Key Laboratory of Biology and Genetic Improvement of Maize (MOA), Beijing Key Laboratory of Crop Genetic Improvement, Joint International Research Laboratory of Crop Molecular Breeding, China Agricultural University, Beijing 100193, China.
Genetics. 2019 Nov;213(3):1065-1078. doi: 10.1534/genetics.119.302594. Epub 2019 Sep 3.
Recombinant inbred lines (RILs) are an important resource for mapping genes controlling complex traits in many species. While RIL populations have been developed for maize, a maize RIL population with multiple teosinte inbred lines as parents has been lacking. Here, we report a teosinte nested association mapping (TeoNAM) population, derived from crossing five teosinte inbreds to the maize inbred line W22. The resulting 1257 BCS RILs were genotyped with 51,544 SNPs, providing a high-density genetic map with a length of 1540 cM. On average, each RIL is 15% homozygous teosinte and 8% heterozygous. We performed joint linkage mapping (JLM) and a genome-wide association study (GWAS) for 22 domestication and agronomic traits. A total of 255 QTL from JLM were identified, with many of these mapping near known genes or novel candidate genes. TeoNAM is a useful resource for QTL mapping for the discovery of novel allelic variation from teosinte. TeoNAM provides the first report that , a rice domestication gene, is also a QTL associated with tillering in teosinte and maize. We detected multiple QTL for flowering time and other traits for which the teosinte allele contributes to a more maize-like phenotype. Such QTL could be valuable in maize improvement.
重组近交系（RILs）是许多物种中控制复杂性状基因图谱的重要资源。虽然已经开发出了用于玉米的 RIL 群体，但缺乏以多种类蜀黍为亲本的玉米 RIL 群体。在这里，我们报告了一个类蜀黍嵌套关联作图（TeoNAM）群体，该群体是通过将五个类蜀黍近交系与玉米近交系 W22 杂交而产生的。由此产生的 1257 个 BCS RIL 用 51544 个 SNP 进行了基因型分析，提供了一个长度为 1540cM 的高密度遗传图谱。平均而言，每个 RIL 是 15%的纯合类蜀黍和 8%的杂合类蜀黍。我们对 22 个驯化和农艺性状进行了联合连锁作图（JLM）和全基因组关联研究（GWAS）。从 JLM 中鉴定出了 255 个 QTL，其中许多 QTL 靠近已知基因或新的候选基因。TeoNAM 是用于 QTL 作图以发现类蜀黍中新型等位基因变异的有用资源。TeoNAM 首次报道，一个水稻驯化基因，也是与类蜀黍和玉米分蘖相关的 QTL。我们检测到多个开花时间和其他性状的 QTL，其中类蜀黍等位基因有助于更类似于玉米的表型。这种 QTL 在玉米改良中可能很有价值。
