Shen Congzhen, Zhang Duoteng, Xu Fang, Yang Yang, Tan Yi, Zhao Qian, Li Lin, Ding Ke, Li Zhengqiu
School of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China.
Key Laboratory of Flexible Electronics (KLOFE), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211800, China.
Biol Chem. 2021 Sep 9;403(4):445-451. doi: 10.1515/hsz-2021-0189. Print 2022 Mar 28.
Thiols are important units in amino acids such as cysteine and peptides like glutathione. Development of chemical sensors capable of precise detection of thiols is important in cancer diagnosis and therapy. We have developed novel two-photon fluorescent turn-on probes for selective detection of thiols. The probes displayed excellent sensitivity and low detection limits. The dual-purpose probes have been demonstrated to be suitable for simultaneous imaging and proteome profiling in live cells and tumor tissues. The unique turn-on design endows the probes with excellent selectivity toward thiols and , and can be further developed to support a thiol-quantification assay.
硫醇是半胱氨酸等氨基酸以及谷胱甘肽等肽类中的重要单元。开发能够精确检测硫醇的化学传感器在癌症诊断和治疗中具有重要意义。我们已经开发出用于选择性检测硫醇的新型双光子荧光开启型探针。这些探针表现出优异的灵敏度和低检测限。已证明这些两用探针适用于活细胞和肿瘤组织中的同步成像和蛋白质组分析。独特的开启型设计赋予探针对硫醇优异的选择性，并且可以进一步开发以支持硫醇定量分析。
