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用于活细胞硫醇检测的荧光探针。

Fluorescent Probes for Live Cell Thiol Detection.

作者信息

Wang Shenggang, Huang Yue, Guan Xiangming

机构信息

Department of Pharmaceutical Sciences, College of Pharmacy and Allied Health Professions, South Dakota State University, Box 2202C, Brookings, SD 57007, USA.

出版信息

Molecules. 2021 Jun 11;26(12):3575. doi: 10.3390/molecules26123575.

DOI:10.3390/molecules26123575
PMID:34208153
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8230801/
Abstract

Thiols play vital and irreplaceable roles in the biological system. Abnormality of thiol levels has been linked with various diseases and biological disorders. Thiols are known to distribute unevenly and change dynamically in the biological system. Methods that can determine thiols' concentration and distribution in live cells are in high demand. In the last two decades, fluorescent probes have emerged as a powerful tool for achieving that goal for the simplicity, high sensitivity, and capability of visualizing the analytes in live cells in a non-invasive way. They also enable the determination of intracellular distribution and dynamitic movement of thiols in the intact native environments. This review focuses on some of the major strategies/mechanisms being used for detecting GSH, Cys/Hcy, and other thiols in live cells via fluorescent probes, and how they are applied at the cellular and subcellular levels. The sensing mechanisms (for GSH and Cys/Hcy) and bio-applications of the probes are illustrated followed by a summary of probes for selectively detecting cellular and subcellular thiols.

摘要

硫醇在生物系统中发挥着至关重要且不可替代的作用。硫醇水平异常与多种疾病和生物功能紊乱有关。已知硫醇在生物系统中分布不均且动态变化。因此，对能够测定活细胞中硫醇浓度和分布的方法有很高的需求。在过去二十年中，荧光探针已成为实现这一目标的强大工具，因为它具有操作简单、灵敏度高以及能够以非侵入性方式在活细胞中可视化分析物的能力。它们还能够在完整的天然环境中测定硫醇的细胞内分布和动态移动。本综述重点介绍了一些通过荧光探针检测活细胞中谷胱甘肽（GSH）、半胱氨酸/同型半胱氨酸（Cys/Hcy）和其他硫醇的主要策略/机制，以及它们在细胞和亚细胞水平上的应用方式。文中阐述了这些探针（针对GSH和Cys/Hcy）的传感机制及生物应用，随后总结了用于选择性检测细胞和亚细胞硫醇的探针。

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