Gladkov Evgeny Aleksandrovich, Gladkova Olga Victorovna
К.А. Timiryazev Institute of Plant Physiology RAS, IPP RAS, 35 Botanicheskaya St., Moscow, 127276, Russia.
, Moscow, Russia.
Environ Sci Pollut Res Int. 2022 Apr;29(16):22947-22951. doi: 10.1007/s11356-021-16355-3. Epub 2021 Sep 10.
Deicing reagents are priority soil pollutants in urban ecosystems. Sodium chloride is one of the priority deicing reagents. Sodium chloride is limiting the spread of lawn grass. We first showed the possibility of using environmental biotechnology in urban greening to obtain lawn grasses tolerant of sodium chloride. We have developed a cell selection technology to obtain salt-tolerant lawn grasses. A cell selection scheme with 1% sodium chloride was used. Most of the tested regenerants were more tolerant to NaCl than original plants. The descendants of the studied regenerants demonstrated the preservation of salt resistance. Most of the descendants of the regenerants Agrostis stolonifera retained high decorative qualities under salinity conditions. The tolerance remained in the next five generations. The descendants of the most salt-tolerant clones Agrostis stolonifera demonstrated resistance to 1% sodium chloride concentration in soil. These plants can serve as the basis for the creation of new salt-tolerant varieties.
除冰剂是城市生态系统中的首要土壤污染物。氯化钠是主要的除冰剂之一。氯化钠限制了草坪草的生长。我们首次展示了在城市绿化中利用环境生物技术获得耐氯化钠草坪草的可能性。我们开发了一种细胞筛选技术来获得耐盐草坪草。使用了含1%氯化钠的细胞筛选方案。大多数测试的再生植株比原始植株更耐氯化钠。所研究的再生植株的后代表现出盐抗性的保留。匍匐翦股颖再生植株的大多数后代在盐分条件下保持了较高的观赏品质。这种耐受性在接下来的五代中依然存在。最耐盐的匍匐翦股颖克隆的后代在土壤中1%氯化钠浓度下表现出抗性。这些植物可作为培育新的耐盐品种的基础。
