Lv Jiangfeng, Wu Yan, Li Wei, Fan Huaping
Department of Clinical Laboratory, Jinan City People's Hospital, Jinan People's Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 271199, China.
Department of Clinical Laboratory, Yantai Yuhuagding Hospital Affiliated to Qingdao University, Yantai, Shandong 264000, China.
J Oncol. 2021 Sep 1;2021:2341719. doi: 10.1155/2021/2341719. eCollection 2021.
Hepatoblastoma (HB) is a kind of tumor that occurs frequently in children and is highly malignant. Here, the function of ROS modulator 1 (ROMO1) was identified in the development of HB. In this study, the mRNA expression of ROMO1 was measured by RT-qPCR. Colony formation assay, MTT assay, and flow cytometric analysis were applied to detect cell viability. The cell migrative and invasive ability was measured by wound healing and transwell assays. Tumor xenografts were performed to examine tumor growth. The results showed that upregulation of ROMO1 was identified in liver hepatocellular carcinoma (LIHC) tissues and predicted poor prognosis in LIHC patients. And ROMO1 expression was also increased in HB tissues and cells. Functionally, ROMO1 knockdown restrained cell viability, migration, and invasion in HB. In addition, knockdown of ROMO1 was found to suppress tumor formation . In conclusion, upregulation of ROMO1 promotes tumor growth and cell aggressiveness in HB.
肝母细胞瘤（HB）是一种在儿童中频繁发生且恶性程度很高的肿瘤。在此，研究了活性氧调节剂1（ROMO1）在HB发生发展中的作用。在本研究中，通过逆转录定量聚合酶链反应（RT-qPCR）检测ROMO1的信使核糖核酸（mRNA）表达。采用集落形成试验、MTT试验和流式细胞术分析检测细胞活力。通过伤口愈合试验和Transwell试验检测细胞的迁移和侵袭能力。进行肿瘤异种移植以检测肿瘤生长情况。结果显示，在肝细胞癌（LIHC）组织中发现ROMO1表达上调，且其表达上调预示LIHC患者预后不良。在HB组织和细胞中，ROMO1表达也增加。在功能上，敲低ROMO1可抑制HB细胞的活力、迁移和侵袭。此外，发现敲低ROMO1可抑制肿瘤形成。总之，ROMO1表达上调促进HB肿瘤生长和细胞侵袭性。
