Department of Anorectal Surgery, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou 215009, China.
First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Biomed Res Int. 2021 Aug 23;2021:6672899. doi: 10.1155/2021/6672899. eCollection 2021.
Slow transit constipation (STC) is characterized by persistent, infrequent, or incomplete defecation. Systematic analyses of mRNA, lncRNA, and circRNA expression profiling in STC provide insights to understand the molecular mechanisms of STC pathogenesis. The present study is aimed at observing the interaction of mRNAs, lncRNAs, and circRNAs by RNA sequencing of STC.
A rat model of STC was induced by loperamide. The expression profiles of both mRNAs and miRNAs were performed by RNA sequencing. Enrichment analyses of anomalous expressed mRNAs, lncRNAs, and circRNAs were performed in order to identify the related biological functions and pathologic pathways through the Gene Ontology (GO) database and Kyoto Encyclopedia of Genes and Genomes (KEGG) database.
In total, 26435 mRNAs, 5703 lncRNAs, and 7708 circRNAs differentially expressed were identified between the two groups. The analyses of GO and KEGG show that (1) upregulated genes were enriched in a positive regulation of GTPase activity, cell migration, and protein binding and lipid binding and (2) GO annotations revealed that most -target mRNAs are involved in the regulation process of immune signal together with the proliferation and differentiation of immune cells. Additionally, the protein-protein interaction (PPI) network of differentially expressed (DE) mRNAs was constructed. Interestingly, all of the core lncRNAs and their coexpression mRNAs in this network are downregulated. Moreover, downregulated circRNAs have a set of target mRNAs related to immunoreaction, which was consistent with the overall tendency.
Our investigation enriches the STC transcriptome database and provides a preliminary exploration of novel candidate genes and avenues expression profiles . The dysregulation of mRNAs, lncRNAs, and circRNAs might contribute to the pathological processes during STC.
慢传输型便秘（STC）的特征为持续、不频繁或不完全排便。对 STC 的 mRNA、lncRNA 和 circRNA 表达谱进行系统分析，有助于了解 STC 发病机制的分子机制。本研究旨在通过 RNA 测序观察 STC 中 mRNA、lncRNA 和 circRNA 的相互作用。
采用洛哌丁胺诱导大鼠 STC 模型，通过 RNA 测序进行 mRNA 和 miRNA 表达谱分析。通过基因本体论（GO）数据库和京都基因与基因组百科全书（KEGG）数据库对异常表达的 mRNAs、lncRNAs 和 circRNAs 进行富集分析，以确定相关的生物学功能和病理途径。
共鉴定出两组间差异表达的 26435 个 mRNAs、5703 个 lncRNAs 和 7708 个 circRNAs。GO 和 KEGG 分析表明，（1）上调基因富集于 GTP 酶活性的正调控、细胞迁移和蛋白结合及脂质结合；（2）GO 注释显示，大多数靶 mRNAs 参与免疫信号的调节过程以及免疫细胞的增殖和分化。此外，构建了差异表达（DE）mRNA 的蛋白质-蛋白质相互作用（PPI）网络。有趣的是，该网络中的所有核心 lncRNAs 及其共表达 mRNAs 均下调。此外，下调的 circRNAs 具有一套与免疫反应相关的靶 mRNAs，这与整体趋势一致。
本研究丰富了 STC 转录组数据库，为新型候选基因和表达谱提供了初步探索。mRNAs、lncRNAs 和 circRNAs 的失调可能有助于 STC 病理过程。
