College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), Nanjing 210095, China.
Mol Plant Microbe Interact. 2021 Dec;34(12):1423-1432. doi: 10.1094/MPMI-07-21-0197-R. Epub 2021 Dec 7.
Alternative splicing of pre-mRNAs is an important gene regulatory mechanism shaping the transcriptome. AtMC1 is an type I metacaspase that positively regulates the hypersensitive response. Here, we found that AtMC1 is involved in the regulation of plant immunity to the bacterial pathogen pv. DC3000 and is physically associated with Sm-like4 (LSM4), which is involved in pre-mRNA splicing. AtMC1 and LSM4 protein levels both increased with their coexpression as compared with their separate expression in vivo. Like AtMC1, LSM4 negatively regulates plant immunity to pv. DC3000 infection. By RNA sequencing, AtMC1 was shown to modulate the splicing of many pre-mRNAs, including , which is a negative regulator of plant immunity. Thus, AtMC1 plays a regulatory role in pre-mRNA splicing, which might contribute to AtMC1-mediated plant immunity.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
前体 mRNA 的可变剪接是一种重要的基因调控机制，可塑造转录组。AtMC1 是一种 I 型类半胱天冬酶，正向调节超敏反应。在这里，我们发现 AtMC1 参与了植物对细菌病原体 pv. DC3000 的免疫调节，并且与参与前体 mRNA 剪接的 Sm 样 4（LSM4）物理相关。AtMC1 和 LSM4 蛋白水平的共表达比单独在体内表达时都增加。与 AtMC1 一样，LSM4 负调控植物对 pv. DC3000 感染的免疫。通过 RNA 测序，AtMC1 被证明可以调节许多前体 mRNA 的剪接，包括 ，它是植物免疫的负调节剂。因此，AtMC1 在 pre-mRNA 剪接中发挥调节作用，这可能有助于 AtMC1 介导的植物免疫。[公式：见文本]版权所有 © 2021 作者。这是一个在 CC BY-NC-ND 4.0 国际许可下发布的开放获取文章。
