Institute of Animal Physiology and Nutrition, Hungarian University of Agricultural and Life Sciences, Kaposvár, Hungary.
Institute of Animal Physiology and Nutrition, Hungarian University of Agricultural and Life Sciences, Herceghalom, Hungary.
PLoS One. 2021 Sep 13;16(9):e0257311. doi: 10.1371/journal.pone.0257311. eCollection 2021.
There are several mathematical models and measurements to determine the efficiency of the digestibility of different feedstuffs. However, there is lack of information regarding the direct methods or measurement techniques used to analyse the physical response of the different parts of the gastrointestinal tract (GIT) of growing pigs to different diets. Smooth muscle electromyography (SMEMG) is a non-invasive method for the measurement of gastrointestinal myoelectrical activity. In the present study, SMEMG methodology has been adapted from laboratory rats to pigs, and the effects of feedstuffs with control (CTR) or experimentally increased (EXP) amounts of fibre were investigated on gastrointestinal tract motility. Nine barrow pigs ((Danish Landrace × Danish Yorkshire) × Danish Duroc) were used (30 ± 3 kg), and their CTR and EXP feedstuffs contained 29 and 49 g/kg crude fibre (CF), respectively. Myoelectric activities of the stomach, ileum and caecum were detected in the awake pigs by a pair of electrodes. The recorded myoelectric signals were analysed with fast Fourier transformation (FFT), and the spectra were expressed in GIT section-specific cycles per minutes (cpm) values and the maximum power spectrum density (PsDmax). A significant increase (P < 0.001) was observed in the value of the PsDmax of the small intestine (20-25 cpm) as a consequence of the EXP diet. The PsDmax values of the stomach (3-5 cpm) and large intestine (1-3 cpm) did not show any significant change in pigs fed the EXP diet. As a direct and non-invasive method, SMEMG is suitable for the rapid evaluation of the effects of diets with different fibre contents on the GIT of non-anaesthetised, free-moving pigs.
有几种数学模型和测量方法可以确定不同饲料的消化效率。然而，关于直接方法或测量技术，用于分析不同饲料对生长猪胃肠道（GIT）不同部位的物理反应的信息却很少。平滑肌肌电图（SMEMG）是一种测量胃肠道肌电活动的非侵入性方法。在本研究中，SMEMG 方法已从实验室大鼠适应到猪，并研究了含有对照（CTR）或实验增加（EXP）纤维量的饲料对胃肠道运动的影响。使用了 9 头阉公猪（（丹麦长白猪×丹麦约克夏猪）×丹麦杜洛克猪）（30±3kg），它们的对照和实验饲料分别含有 29 和 49g/kg 粗饲料（CF）。通过一对电极在清醒的猪中检测胃、回肠和盲肠的肌电活动。记录的肌电信号用快速傅里叶变换（FFT）进行分析，频谱以胃肠道节段特定的每分钟周期数（cpm）值和最大功率谱密度（PsDmax）表示。由于 EXP 饮食，小肠（20-25cpm）的 PsDmax 值显著增加（P<0.001）。用 EXP 饮食喂养的猪的胃（3-5cpm）和大肠（1-3cpm）的 PsDmax 值没有显示出任何显著变化。作为一种直接和非侵入性的方法，SMEMG 适合快速评估不同纤维含量的饮食对非麻醉、自由活动猪的胃肠道的影响。
