Fifel Karim, Deboer Tom
Laboratory for Neurophysiology, Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, The Netherlands.
Yanagisawa Lab, International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba, Ibaraki, Japan.
Sleep. 2022 Jan 11;45(1). doi: 10.1093/sleep/zsab230.
Basal ganglia (BG) are a set of subcortical nuclei that are involved in the control of a wide variety of motor, cognitive, and affective behaviors. Although many behavioral abnormalities associated with BG dysfunction overlap with the clinical picture precipitated by the lack of sleep, the impact of sleep alterations on neuronal activity in BG is unknown. Using wild-type C57BI mice, we investigated the circadian and sleep-related homeostatic modulation of neuronal activity in the three functional subdivisions of the striatum (i.e. sensorimotor, associative, and limbic striatum). We found no circadian modulation of activity in both ventral and dorsomedial striatum while the dorsolateral striatum displayed a significant circadian rhythm with increased firing rates during the subjective dark, active phase. By combining neuronal activity recordings with electroencephalogram (EEG) recordings, we found a strong modulation of neuronal activity by the nature of vigilance states with increased activity during wakefulness and rapid eye movement sleep relative to nonrapid eye movement sleep in all striatal subregions. Depriving animals of sleep for 6 h induced significant, but heterogenous alterations in the neuronal activity across striatal subregions. Notably, these alterations lasted for up to 48 h in the sensorimotor striatum and persisted even after the normalization of cortical EEG power densities. Our results show that vigilance and sleep states as well as their disturbances significantly affect neuronal activity within the striatum. We propose that these changes in neuronal activity underlie both the well-established links between sleep alterations and several disorders involving BG dysfunction as well as the maladaptive changes in behavior induced in healthy participants following sleep loss.
基底神经节（BG）是一组皮层下核团，参与多种运动、认知和情感行为的控制。尽管许多与BG功能障碍相关的行为异常与睡眠不足引发的临床症状重叠，但睡眠改变对BG神经元活动的影响尚不清楚。我们使用野生型C57BI小鼠，研究了纹状体三个功能分区（即感觉运动、联合和边缘纹状体）中神经元活动的昼夜节律和与睡眠相关的稳态调节。我们发现腹侧和背内侧纹状体的活动没有昼夜节律调节，而背外侧纹状体显示出显著的昼夜节律，在主观黑暗的活跃期放电率增加。通过将神经元活动记录与脑电图（EEG）记录相结合，我们发现所有纹状体亚区域中，警觉状态的性质对神经元活动有强烈调节作用，相对于非快速眼动睡眠，清醒和快速眼动睡眠期间活动增加。剥夺动物6小时睡眠会导致纹状体亚区域神经元活动出现显著但异质性的改变。值得注意的是，这些改变在感觉运动纹状体中持续长达48小时，即使皮质EEG功率密度恢复正常后仍持续存在。我们的结果表明，警觉和睡眠状态及其干扰会显著影响纹状体内的神经元活动。我们提出，这些神经元活动的变化是睡眠改变与几种涉及BG功能障碍的疾病之间既定联系的基础，也是健康参与者睡眠剥夺后行为中适应不良变化的基础。
