School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230026, PR China; Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science, Suzhou, 215163, PR China.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science, Suzhou, 215163, PR China.
Talanta. 2021 Dec 1;235:122770. doi: 10.1016/j.talanta.2021.122770. Epub 2021 Aug 4.
2D materials with attractive optical properties are promising for individualized cancer immunotherapy. Isolation, capture, and release of circulating tumor cells (CTCs) are of great significance for promoting the process of early diagnosis of cancers. MXene nanosheets incorporated gelatin hydrogel offers the possibility of achieving near-infrared (NIR) light response to initiate the photothermal effect. Herein, the design and preparation of TiCT MXene nanosheets-embedded thermoresponsive gelatin hydrogel membrane with NIR light-responsive for the specific capture and release of CTCs were reported. The membrane was fabricated by casting TiCT-embedded gelatin onto a substrate and then modified with epithelial-cell adhesion-molecule antibody (anti-EpCAM) for the specific recognition and separation of CTCs from whole blood. The captured cells can be released without damage with dual-mode containing temperature-responsive release (gelatin deconstructed at 37 °C) and photothermal site-release (TiCT induced by NIR light). Furthermore, we were able to achieve an average efficient release rate of 89 % of captured cells with stable cell viability of 87 % via the NIR light irradiation. This work may provide the promising potential for retrieval of single cells in clinical diagnosis.
具有诱人光学特性的二维材料有望应用于个体化癌症免疫治疗。分离、捕获和释放循环肿瘤细胞（CTCs）对于促进癌症的早期诊断过程具有重要意义。将 MXene 纳米片掺入明胶水凝胶中提供了实现近红外（NIR）光响应以引发光热效应的可能性。在此，报告了一种设计和制备 TiCT MXene 纳米片嵌入的温敏明胶水凝胶膜的方法，该膜具有 NIR 光响应特性，可用于特定捕获和释放 CTCs。该膜是通过将 TiCT 嵌入的明胶浇铸在基底上，然后用上皮细胞黏附分子抗体（anti-EpCAM）修饰而成，用于从全血中特异性识别和分离 CTCs。捕获的细胞可以通过双模式释放，包括温度响应释放（在 37°C 下明胶解构）和光热位点释放（NIR 光诱导的 TiCT），而不会造成损伤。此外，通过 NIR 光照射，我们能够实现 89%的捕获细胞的平均有效释放率，同时保持 87%的稳定细胞活力。这项工作可能为临床诊断中单个细胞的回收提供了有前途的潜力。
