伴 Tfh 细胞改变的 B 细胞在儿童过敏性紫癜中的变化。
Altered B cell compartment associated with Tfh cells in children with Henoch-Schonlein Purpura.
机构信息
Department of Hematology, The First Affiliated Hospital of Jinzhou Medical University, No.2 Renmin Street, Liaoning, 121000, Jinzhou, People's Republic of China.
出版信息
BMC Pediatr. 2021 Sep 13;21(1):399. doi: 10.1186/s12887-021-02873-z.
AIM
IgA-producing B cells have been found to be associated with children diagnosed with Henoch-Schonlein purpura (HSP). The aim of the present study was to determine whether children with HSP possess altered B-cell subsets.
METHODS
A total of 14 children diagnosed with HSP and age- and sex-matched healthy controls (HCs) were enrolled in our study. Peripheral blood mononuclear cells were isolated, and the percentage and absolute number of B-cell subsets and Follicular helper T (Tfh) cells were determined by flow cytometry. Finally, Spearman's correlation coefficient was used to analyse the correlation between the percentage of Tfh cells and B-cell subsets.
RESULTS
We found that compared to HCs, the frequency and absolute number of total B cells were significantly higher in children with HSP, but the percentages of plasma cells and naïve B cells were significantly lower. A significantly increased percentage and absolute number of memory nonswitched B cells were found in children with HSP compared with HCs. We observed that the expression of C-X-C chemokine receptor type 5 (CXCR5) on total CD4 T cells and the percentage of CD4CXCR5 cells were significantly increased in patients with HSP. Moreover, significantly correlations between Tfh cells and various B-cell subsets were observed.
CONCLUSIONS
Our study showed a Tfh-cell-associated altered B cell compartment in children with HSP.
目的
已发现 IgA 产生 B 细胞与诊断为过敏性紫癜（HSP）的儿童有关。本研究旨在确定 HSP 患儿是否存在改变的 B 细胞亚群。
方法
本研究纳入了 14 名 HSP 患儿和年龄及性别匹配的健康对照者（HCs）。通过流式细胞术分离外周血单个核细胞，并确定 B 细胞亚群和滤泡辅助 T（Tfh）细胞的百分比和绝对数。最后，采用 Spearman 相关系数分析 Tfh 细胞与 B 细胞亚群之间的相关性。
结果
与 HCs 相比，HSP 患儿的总 B 细胞频率和绝对数显著升高，但浆细胞和幼稚 B 细胞的百分比显著降低。与 HCs 相比，HSP 患儿的记忆性未转换 B 细胞的频率和绝对数显著增加。我们观察到 HSP 患者的总 CD4 T 细胞上 C-X-C 趋化因子受体 5（CXCR5）的表达和 CD4^+CXCR5^+细胞的百分比显著增加。此外，还观察到 Tfh 细胞与各种 B 细胞亚群之间存在显著相关性。
结论
本研究显示 HSP 患儿存在 Tfh 细胞相关的 B 细胞亚群改变。
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