Wang Bin, Shao-Kuan Fu-Rong, Dong Chen
Zhongguo Dang Dai Er Ke Za Zhi. 2020 May;22(5):473-477. doi: 10.7499/j.issn.1008-8830.1911029.
To study the role of follicular helper T (Tfh) cells and galactose-deficient IgA1 (Gd-IgA1) in the pathogenesis of childhood Henoch-Schönlein purpura (HSP) and the correlation between them.
A total of 36 children with newly-diagnosed HSP were enrolled. They were divided into two groups: HSP nephritis (HSPN) group with 11 children and non-HSPN group with 25 children according to the presence or absence of HSPN. Another 15 children who underwent physical examination at the outpatient service were enrolled as the healthy control group. Flow cytometry was used to measure the proportion of Tfh cells (CD4CXCR5ICOS) in peripheral blood. ELISA was used to measure the levels of interleukin-21 (IL-21) and interleukin-6 (IL-6) in peripheral blood and the serum levels of IgA1 and Gd-IgA1. A Pearson correlation analysis was used to investigate the correlation of serum Gd-IgA1 concentration with Tfh cells and related factors expression in the children with HSP.
Both the HSPN and non-HSPN groups had significantly higher proportion of Tfh cells and expression levels of IL-21 and IL-6 in peripheral blood than the healthy control group (P<0.05). The HSPN group had significant increases in the above indices compared with the non-HSPN group (P<0.05). Both the HSPN and non-HSPN groups had significantly higher serum levels of IgA1 and Gd-IgA1 than the healthy control group (P<0.05). The HSPN group had significantly higher serum levels of IgA1 and Gd-IgA1 than the non-HSPN group (P<0.05). In the children with HSP, serum Gd-IgA1 level was positively correlated with Tfh cells proportion and IL-21 and IL-6 levels (P<0.05).
Tfh cells and related cytokines and serum Gd-IgA1 are involved in the development of HSP/HSPN. Tfh cells may mediate the increased production of Gd-IgA1.
探讨滤泡辅助性T（Tfh）细胞及缺乏半乳糖的IgA1（Gd-IgA1）在儿童过敏性紫癜（HSP）发病机制中的作用及其二者之间的相关性。
选取36例新诊断的HSP患儿，根据是否合并HSP肾炎（HSPN）分为两组：HSPN组11例，非HSPN组25例。另选取15例门诊体检儿童作为健康对照组。采用流式细胞术检测外周血中Tfh细胞（CD4CXCR5ICOS）比例。采用酶联免疫吸附测定（ELISA）法检测外周血白细胞介素-21（IL-21）、白细胞介素-6（IL-6）水平及血清IgA1和Gd-IgA1水平。采用Pearson相关分析探讨HSP患儿血清Gd-IgA1浓度与Tfh细胞及相关因子表达的相关性。
HSPN组和非HSPN组外周血Tfh细胞比例、IL-21及IL-6表达水平均显著高于健康对照组（P<0.05）。HSPN组上述指标较非HSPN组显著升高（P<0.05）。HSPN组和非HSPN组血清IgA1和Gd-IgA1水平均显著高于健康对照组（P<0.05）。HSPN组血清IgA1和Gd-IgA1水平显著高于非HSPN组（P<0.05）。HSP患儿血清Gd-IgA1水平与Tfh细胞比例、IL-21及IL-6水平呈正相关（P<0.05）。
Tfh细胞及相关细胞因子和血清Gd-IgA1参与HSP/HSPN的发病过程。Tfh细胞可能介导Gd-IgA1产生增加。
