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1
Expression of neuronal acetylcholine receptor polypeptides in vitro.神经元乙酰胆碱受体多肽的体外表达。
Cell Mol Neurobiol. 1987 Dec;7(4):391-401. doi: 10.1007/BF00733791.
2
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Distribution of acetylcholine receptors in the central nervous system of adult locusts.成年蝗虫中枢神经系统中乙酰胆碱受体的分布
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5
Characterization of the mRNA for mouse muscle acetylcholine receptor alpha subunit by quantitative translation in vitro.通过体外定量翻译对小鼠肌肉乙酰胆碱受体α亚基的mRNA进行表征。
J Biol Chem. 1983 Mar 10;258(5):3294-303.
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In vitro synthesis of laminin and entactin polypeptides.层粘连蛋白和巢蛋白多肽的体外合成
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In vitro synthesis of polypeptides of moderately large size by poly(A)-containing messenger RNA from postmortem human brain and mouse brain.利用来自人死后大脑和小鼠大脑的含聚腺苷酸信使核糖核酸体外合成中等大小的多肽。
J Neurochem. 1983 Mar;40(3):670-80. doi: 10.1111/j.1471-4159.1983.tb08032.x.
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Primary structures of beta- and delta-subunit precursors of Torpedo californica acetylcholine receptor deduced from cDNA sequences.从cDNA序列推导的加州电鳐乙酰胆碱受体β和δ亚基前体的一级结构。
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Immunocytochemical localization of acetylcholine receptors in locust brain using auto-anti-idiotypic acetylcholine antibodies.利用自身抗独特型乙酰胆碱抗体对蝗虫大脑中乙酰胆碱受体进行免疫细胞化学定位。
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本文引用的文献

1
In vitro synthesis, glycosylation, and membrane insertion of the four subunits of Torpedo acetylcholine receptor.电鳐乙酰胆碱受体四个亚基的体外合成、糖基化及膜插入
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5598-602. doi: 10.1073/pnas.78.9.5598.
2
Isolation of a putative nicotinic acetylcholine receptor from the central nervous system of Locusta migratoria.从飞蝗中枢神经系统中分离出一种假定的烟碱型乙酰胆碱受体。
Neurosci Lett. 1984 May 18;46(3):323-8. doi: 10.1016/0304-3940(84)90119-8.
3
The regulation of acetylcholine receptor expression in mammalian muscle.哺乳动物肌肉中乙酰胆碱受体表达的调控。
Cold Spring Harb Symp Quant Biol. 1983;48 Pt 1:135-46. doi: 10.1101/sqb.1983.048.01.016.
4
Molecular events in the synthesis and assembly of a nicotinic acetylcholine receptor.烟碱型乙酰胆碱受体合成与组装中的分子事件
Cold Spring Harb Symp Quant Biol. 1983;48 Pt 1:125-34. doi: 10.1101/sqb.1983.048.01.015.
5
Identification of homo-oligomers as potential intermediates in acetylcholine receptor subunit assembly.鉴定同型寡聚体作为乙酰胆碱受体亚基组装中的潜在中间体。
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4359-63. doi: 10.1073/pnas.80.14.4359.
6
Nicotinic receptor of acetylcholine: structure of an oligomeric integral membrane protein.乙酰胆碱的烟碱型受体：一种寡聚整合膜蛋白的结构
Physiol Rev. 1984 Oct;64(4):1162-239. doi: 10.1152/physrev.1984.64.4.1162.
7
Synthesis in vitro of precursors of the catalytic subunits of acetylcholinesterase from Torpedo marmorata and Electrophorus electricus.电鳐和电鳗乙酰胆碱酯酶催化亚基前体的体外合成
Eur J Biochem. 1984 Dec 17;145(3):519-24. doi: 10.1111/j.1432-1033.1984.tb08587.x.
8
In vitro synthesis of polypeptides of moderately large size by poly(A)-containing messenger RNA from postmortem human brain and mouse brain.利用来自人死后大脑和小鼠大脑的含聚腺苷酸信使核糖核酸体外合成中等大小的多肽。
J Neurochem. 1983 Mar;40(3):670-80. doi: 10.1111/j.1471-4159.1983.tb08032.x.
9
A system for the translation of receptor messenger-RNA and the study of the assembly of functional receptors.一种用于翻译受体信使核糖核酸及研究功能性受体组装的系统。
J Recept Res. 1984;4(1-6):681-704. doi: 10.3109/10799898409042581.
10
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.

神经元乙酰胆碱受体多肽的体外表达。

Expression of neuronal acetylcholine receptor polypeptides in vitro.

作者信息

Benke D, Breer H

机构信息

Department of Zoophysiology, University of Osnabrück, West Germany.

出版信息

Cell Mol Neurobiol. 1987 Dec;7(4):391-401. doi: 10.1007/BF00733791.

DOI:10.1007/BF00733791
PMID:3451797
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11567213/
Abstract
  1. Translation of poly(A) RNA extracted from the nervous tissue of locusts in a reticulocyte lysate system led to polypeptides with a broad spectrum of molecular weights. 2. Using anti-locust acetylcholine receptor (AChR) antisera, polypeptides with a molecular weight of about 50,000 were immunoprecipitated. These peptides comprised about 0.3% of the total translation products. 3. Cotranslational incubation with pancreatic rough microsomes resulted in a glycosylated 60,000-dalton immunoprecipitate. 4. Density-gradient analysis of in vitro synthesized and glycosylated receptor polypeptides indicated that no assembly of subunits had taken the place under the in vitro conditions.
摘要
  1. 在网织红细胞裂解物系统中对从蝗虫神经组织提取的聚腺苷酸RNA进行翻译，产生了分子量范围广泛的多肽。2. 使用抗蝗虫乙酰胆碱受体（AChR）抗血清，对分子量约为50,000的多肽进行了免疫沉淀。这些肽约占总翻译产物的0.3%。3. 与胰腺粗面微粒体进行共翻译孵育，得到了一种糖基化的60,000道尔顿免疫沉淀物。4. 对体外合成和糖基化的受体多肽进行密度梯度分析表明，在体外条件下没有发生亚基组装。