神经元乙酰胆碱受体多肽的体外表达。
Expression of neuronal acetylcholine receptor polypeptides in vitro.
作者信息
Benke D, Breer H
机构信息
Department of Zoophysiology, University of Osnabrück, West Germany.
出版信息
Cell Mol Neurobiol. 1987 Dec;7(4):391-401. doi: 10.1007/BF00733791.
Abstract
- Translation of poly(A) RNA extracted from the nervous tissue of locusts in a reticulocyte lysate system led to polypeptides with a broad spectrum of molecular weights. 2. Using anti-locust acetylcholine receptor (AChR) antisera, polypeptides with a molecular weight of about 50,000 were immunoprecipitated. These peptides comprised about 0.3% of the total translation products. 3. Cotranslational incubation with pancreatic rough microsomes resulted in a glycosylated 60,000-dalton immunoprecipitate. 4. Density-gradient analysis of in vitro synthesized and glycosylated receptor polypeptides indicated that no assembly of subunits had taken the place under the in vitro conditions.
摘要
- 在网织红细胞裂解物系统中对从蝗虫神经组织提取的聚腺苷酸RNA进行翻译，产生了分子量范围广泛的多肽。2. 使用抗蝗虫乙酰胆碱受体（AChR）抗血清，对分子量约为50,000的多肽进行了免疫沉淀。这些肽约占总翻译产物的0.3%。3. 与胰腺粗面微粒体进行共翻译孵育，得到了一种糖基化的60,000道尔顿免疫沉淀物。4. 对体外合成和糖基化的受体多肽进行密度梯度分析表明，在体外条件下没有发生亚基组装。
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本文引用的文献
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Proc Natl Acad Sci U S A. 1981 Sep;78(9):5598-602. doi: 10.1073/pnas.78.9.5598.
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