Sikorav J L, Grassi J, Bon S
Eur J Biochem. 1984 Dec 17;145(3):519-24. doi: 10.1111/j.1432-1033.1984.tb08587.x.
We translated poly(A-rich messenger RNA prepared from the electric organs of Electrophorus electricus and Torpedo marmorata in a reticulocyte lysate system. In the case of Electrophorus, which appears to contain only one type of acetylcholinesterase catalytic subunit, an anti-(Electrophorus acetylcholinesterase) antiserum precipitated a single 65-kDa polypeptide from the products translation obtained in vitro. In the case of Torpedo, where a number of distinct catalytic subunits corresponding to different fractions of the enzyme have been described, an anti-(Torpedo acetylcholinesterase) antiserum precipitated two main polypeptides, 61 kDa and 65 kDa, both of which could be displaced by unlabelled purified Torpedo acetylcholinesterase. Synthesis in vitro thus appears to produce a single type of precursor of the acetylcholinesterase catalytic subunit for Electrophorus, and at least two distinct precursors for Torpedo, suggesting that several mRNAs code for the catalytic subunits in the latter species.
我们在网织红细胞裂解物系统中翻译了从电鳗（Electrophorus electricus）和斑纹电鲼（Torpedo marmorata）的电器官制备的富含多聚腺苷酸的信使核糖核酸。就电鳗而言，它似乎只含有一种乙酰胆碱酯酶催化亚基，一种抗（电鳗乙酰胆碱酯酶）抗血清从体外获得的翻译产物中沉淀出一条单一的65 kDa多肽。就斑纹电鲼而言，已经描述了与该酶不同组分相对应的多种不同催化亚基，一种抗（斑纹电鲼乙酰胆碱酯酶）抗血清沉淀出两条主要多肽，61 kDa和65 kDa，这两条多肽都可以被未标记的纯化斑纹电鲼乙酰胆碱酯酶取代。因此，体外合成似乎为电鳗产生了一种单一类型的乙酰胆碱酯酶催化亚基前体，而为斑纹电鲼产生了至少两种不同的前体，这表明在后者物种中有几种信使核糖核酸编码催化亚基。
