Fisher W F, Guillot F S, Cole N A
U.S. Livestock Insects Laboratory, Agricultural Research Service, USDA, Kerrville, TX 78029-0232.
Exp Appl Acarol. 1986 Sep;2(3):239-48. doi: 10.1007/BF01193956.
The serum antibody activity to Psoroptes ovis antigens, as determined by enzyme-linked immunosorbent assay (ELISA), was monitored during the development and decline phase of scabies in cattle herds under feedlot conditions in an endemic (Bushland) and a nonendemic (Kerrville) scabies area of Texas. The infestations were initiated by exposing six calves in each of the herds to P. ovis and allowing the infestations to spread to other members of the herd. The cattle in the endemic area developed more lesions and a higher incidence of the disease than did the cattle in the nonendemic area; also, there were more cattle with specific serum antibody activity in the Bushland herd than in the Kerrville herd at the peak of the infestations (late January--early February). As infestations declined because of summer latency, the number of calves with specific serum antibody activity declined in both herds. Specific serum antibody activity disappeared in the Kerrville cattle when P. ovis was no longer detected. However, specific serum antibody activity was still detected in some of the Bushland cattle in late summer (August) and fall (October) examinations, when low numbers of P. ovis were detected. These results indicate that the ELISA would be very useful in detection of specific serum antibody activity in lightly infested calves, especially during the developmental and decline phases of the disease.
在得克萨斯州一个疥疮流行地区（布什兰）和一个非流行地区（克尔维尔）的饲养场条件下，对牛群疥螨病的发生和消退阶段，采用酶联免疫吸附测定（ELISA）法监测血清中对绵羊痒螨抗原的抗体活性。通过让每个牛群中的6头犊牛接触绵羊痒螨并使其在牛群中传播来引发感染。流行地区的牛比非流行地区的牛出现更多病变和更高的发病率；此外，在感染高峰期（1月下旬至2月初），布什兰牛群中具有特异性血清抗体活性的牛比克尔维尔牛群中的更多。随着夏季潜伏期导致感染减少，两个牛群中具有特异性血清抗体活性的犊牛数量均下降。当在克尔维尔牛中不再检测到绵羊痒螨时，特异性血清抗体活性消失。然而，在夏末（8月）和秋季（10月）检查时，当检测到少量绵羊痒螨时，在一些布什兰牛中仍检测到特异性血清抗体活性。这些结果表明，ELISA在检测轻度感染犊牛的特异性血清抗体活性方面非常有用，尤其是在疾病的发展和消退阶段。
