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定量单管酶联免疫吸附测定的动力学研究

Kinetic studies of a quantitative single-tube enzyme-linked immunosorbent assay.

作者信息

Tsang V C, Wilson B C, Maddison S E

出版信息

Clin Chem. 1980 Aug;26(9):1255-60.

PMID:6772340
Abstract

We describe a single-cuvette enzyme-linked immunosorbent assay (ELISA) based on enzymic rate kinetics. This kinetic assay can yield linear quantitative data on immunoglobin concentrations. Optimum assay conditions, component concentrations, reaction intervals, and pH are described. Assay linearity and sensitivity are demonstrated in systems involving immunoglobulin G and antibodies to it, and with pooled sera from monkeys infected with Schistosoma mansoni.

摘要

我们描述了一种基于酶促速率动力学的单比色皿酶联免疫吸附测定（ELISA）。这种动力学测定法能够得出关于免疫球蛋白浓度的线性定量数据。文中描述了最佳测定条件、组分浓度、反应间隔和pH值。在涉及免疫球蛋白G及其抗体的系统中，以及在感染曼氏血吸虫的猴子的混合血清中，证明了测定的线性和灵敏度。

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1
Kinetic studies of a quantitative single-tube enzyme-linked immunosorbent assay.定量单管酶联免疫吸附测定的动力学研究
Clin Chem. 1980 Aug;26(9):1255-60.
2
Applicability of different antigen preparations in the enzyme-linked immunosorbent assay for schistosomiasis mansoni.不同抗原制剂在曼氏血吸虫病酶联免疫吸附试验中的适用性。
Am J Trop Med Hyg. 1980 May;29(3):401-10. doi: 10.4269/ajtmh.1980.29.401.
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Am J Trop Med Hyg. 1980 Jan;29(1):82-5. doi: 10.4269/ajtmh.1980.29.82.
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Serological differences between acute and chronic schistosomiasis mansoni detected by enzyme-linked immunosorbent assay (ELISA).
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Detection of antibodies against circulating cathodic antigen of Schistosoma mansoni using the enzyme-linked immunosorbent assay.
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Standardization of FIAXtm for schistosomiasis using crude cercarial and adult worm antigens.使用粗制尾蚴和成虫抗原对用于血吸虫病的FIAXtm进行标准化。
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Cross-linking of Schistosoma mansoni antigens and their covalent binding on the surface of polystyrene microtitration trays for use in the ELISA.
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[Detection of antibodies against ovum antigens in Schistosoma mansoni bilharziasis].
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