Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
Cancer Center, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, China.
Nanoscale. 2021 Oct 21;13(40):16923-16931. doi: 10.1039/d1nr04112f.
Immunomagnetic beads have been widely explored as an important analytical tool for the rapid and sensitive detection of circulating tumor cells (CTCs). However, their clinical application is seriously hindered by the tedious preparation procedures and heterogeneous nature of CTCs. To this end, a designed multifunctional platform named FeO@TA/Cu superparamagnetic nanoparticles (SPMNPs) is expected to have the following features: (i) the formation of a tannic acid-copper (II) ion (TA/Cu) coating which could be accomplished by a one-step method is very simple; (ii) the TA/Cu coating shows high affinity for heterogeneous CTCs and good resistance to nonspecific adhesion of blood cells; (iii) "self-release" of the captured cells could be achieved as the TA/Cu coating gradually degrades in the cell culture environment without any additional interventions. Therefore, the resulting FeO@TA/Cu SPMNPs could capture various CTCs (MCF-7, HepG2 and HeLa cells) with different expression levels of the epithelial cell adhesion molecule (EpCAM). And the capture efficiency and cell purity can reach 88% and 87%, respectively. In addition, 68% of the captured cells are self-released after 6 h of incubation and most of the released cells show high cell proliferation activity. In particular, FeO@TA/Cu SPMNPs can successfully detect 1-13 CTCs from 1 mL of blood of 14 patients with 6 types of cancers. Hence, we expect that the as-prepared FeO@TA/Cu SPMNPs with simple, efficient, and universal yet cost-efficient characteristics could act as a promising analytical tool for clinical CTC detection.
免疫磁珠已被广泛探索作为一种用于快速和灵敏检测循环肿瘤细胞（CTC）的重要分析工具。然而，其临床应用受到 CTC 制备程序繁琐和异质性的严重阻碍。为此，设计了一种名为 FeO@TA/Cu 超顺磁纳米粒子（SPMNPs）的多功能平台，具有以下特点：（i）通过一步法形成单宁酸-铜（II）离子（TA/Cu）涂层非常简单；（ii）TA/Cu 涂层对异质 CTC 具有高亲和力，并且对血细胞的非特异性黏附具有良好的抵抗力；（iii）在细胞培养环境中，TA/Cu 涂层逐渐降解时，可以实现捕获细胞的“自释放”，而无需任何额外的干预。因此，所得的 FeO@TA/Cu SPMNPs 可以捕获具有不同上皮细胞黏附分子（EpCAM）表达水平的各种 CTC（MCF-7、HepG2 和 HeLa 细胞）。捕获效率和细胞纯度分别达到 88%和 87%。此外，孵育 6 小时后，68%的捕获细胞自释放，并且大部分释放的细胞显示出高细胞增殖活性。特别地，FeO@TA/Cu SPMNPs 可以从 14 名患有 6 种癌症的患者的 1 毫升血液中成功检测到 1-13 个 CTC。因此，我们期望具有简单、高效、通用且成本效益高的特点的所制备的 FeO@TA/Cu SPMNPs 可以作为一种有前途的临床 CTC 检测分析工具。
