School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei 230026, China.
CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
ACS Appl Mater Interfaces. 2021 Jan 27;13(3):3694-3700. doi: 10.1021/acsami.0c20916. Epub 2021 Jan 14.
The majority of current methods of isolating circulating tumor cells (CTCs) rely on a biomarker. However, the isolation efficiency may be compromised due to the heterogeneity of CTCs. In this work, a simple and broad-spectrum method is established to efficiently isolate the heterogeneous CTCs from patient blood samples using tannic acid (TA)-functionalized magnetic nanoparticles (MNPs). The TA-functionalized MNPs (MNPs-TA) inhibit the nonspecific adhesion of peripheral blood mononuclear cell (PBMC) and enhance cancer cell capture, resulting from the unique interaction between TA and glycocalyx on cancer cells. The MNPs-TA was demonstrated to effectively capture seven kinds of cancer cells (HeLa, PC-3, T24, MAD-MB-231, MCF-7, HT1080, A549) from artificial samples (62.3-93.7%). Moreover, this epithelial cell adhesion molecule (EpCAM)-independent CTC isolation method was also tested using clinical blood samples from patients with different cancers (21 patients), which may provide a universal tool to detect CTCs in the clinic.
目前大多数分离循环肿瘤细胞（CTC）的方法都依赖于生物标志物。然而，由于 CTC 的异质性，其分离效率可能会受到影响。在这项工作中，建立了一种简单且广谱的方法，使用单宁酸（TA）功能化的磁性纳米颗粒（MNPs）从患者的血液样本中高效分离异质 CTC。TA 功能化的 MNPs（MNPs-TA）通过 TA 与癌细胞糖萼之间的独特相互作用，抑制外周血单核细胞（PBMC）的非特异性黏附，并增强癌细胞的捕获能力，从而抑制 PBMC 的非特异性黏附并增强癌细胞的捕获能力。MNPs-TA 被证明能够从人工样本（62.3-93.7%）中有效捕获七种癌细胞（HeLa、PC-3、T24、MAD-MB-231、MCF-7、HT1080、A549）。此外，还使用来自不同癌症患者（21 名患者）的临床血液样本测试了这种不依赖于上皮细胞黏附分子（EpCAM）的 CTC 分离方法，这可能为临床检测 CTC 提供了一种通用工具。
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