Monthly rather than annual climate variation determines plant diversity change in four temperate grassland nature reserves.

Plant diversity is changing in the world; climate variation at annual scale is believed to drive these changes; however, the effects of climate variation at month scale are still unknown. Anxi, West Ordos, Xilingol, and Tumuji grassland nature reserves, located in northern China, have been well protected from human disturbance, are ideal areas to identify the drive forces for plant diversity change. Using Landsat images from 1982 to 2017, we analyzed the evolution of month- and annual-climate variables and spectral plant diversity indices, and explored the effects of the variability of temperature and precipitation on plant diversity and their relationship. The results showed that the diversity of the four grasslands was decreasing. Climate variables, in particular temperature at month scale, significantly related to grassland plant diversity. These results enlarge our understanding in how climate change driving plant diversity during a long term. Measurements coping with plant diversity decreasing may be more effective and earlier based on monthly climate variables.