Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, 213000, Jiangsu Province, PR China.
Epigenomics. 2021 Aug;13(16):1281-1297. doi: 10.2217/epi-2021-0130. Epub 2021 Sep 15.
We aimed to explore the effect of long noncoding RNA in colorectal cancer (CRC). Relative gene and protein expression were screened. Colony formation and flow cytometry assays were performed to determine proliferation and apoptosis. Dual luciferase and RNA immunoprecipitation assays were conducted to validate the interaction between indicated molecules. Xenograft in nude mice was applied to verify the conclusion . and PD-L1 were upregulated while miR-20b-5p was downregulated in CRC tissue. Functional analysis revealed that lncRNA HCG18 promoted proliferation, migration and resistance to cetuximab of CRC cells via the miR-20b-5p/PD-L1 axis. facilitated progress of the tumor, conferred to cetuximab resistance and suppressed CD8 T cells via the miR-20b-5p/PD-L1 axis.
我们旨在探讨长链非编码 RNA 在结直肠癌（CRC）中的作用。筛选相对基因和蛋白表达。进行集落形成和流式细胞术分析以确定增殖和凋亡。双荧光素酶和 RNA 免疫沉淀测定验证指示分子之间的相互作用。裸鼠移植瘤实验验证结论。在 CRC 组织中，HCG18 上调，miR-20b-5p 下调，而 PD-L1 上调。功能分析表明，lncRNA HCG18 通过 miR-20b-5p/PD-L1 轴促进 CRC 细胞的增殖、迁移和对西妥昔单抗的耐药性。HCG18 通过 miR-20b-5p/PD-L1 轴促进肿瘤进展，赋予西妥昔单抗耐药性并抑制 CD8 T 细胞。
