Beijing Fisheries Research Institute, Beijing Academy of Agricultural and Forestry Sciences, Beijing, China.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
J Sci Food Agric. 2022 Mar 30;102(5):1948-1957. doi: 10.1002/jsfa.11532. Epub 2021 Sep 27.
Recent studies demonstrate that fish byproducts can be used as sources of bioactive peptides for functional foods. Sturgeon skin contains abundant proteins but it has commonly been discarded during sturgeon processing. The objective of the present work was to identify and characterize the bioactive peptides from protein hydrolysates of sturgeon skin.
Sturgeon skin protein extract (SKPE) hydrolyzed by flavourzyme for 60 min exhibited high antioxidant activity, dipeptidyl peptidase IV (DPP-IV) and angiotensin converting enzyme (ACE) inhibitory activity. The sequences of peptides from flavourzyme hydrolysates were identified using high-performance liquid chromatography-tandem mass spectrometry. Gly-Asp-Arg-Gly-Glu-Ser-Gly-Pro-Ala (P1) showed the highest DPPH radical scavenging activity (DPPH IC = 1.93 mmol L ). Gly-Pro-Ala-Gly-Glu-Arg-Gly-Glu-Gly-Gly-Pro-Arg (P11) (DPP-IV IC = 2.14 mmol L ) and Ser-Pro-Gly-Pro-Asp-Gly-Lys-Thr-Gly-Pro-Arg (P12) (DPP-IV IC = 2.61 mmol L ) exhibited the strongest DPP-IV inhibitory activity. Gly-Pro-Pro-Gly-Ala-Asp-Gly-Gln-Ala-Gly-Ala-Lys (P6) displayed the highest ACE inhibitory activity (ACE IC = 3.77 mmol L ). The molecular docking analysis revealed that DPP-IV inhibition of P11 and P12 are mainly attributed to hydrogen bonds and hydrophobic interactions, whereas ACE inhibition of P6 is mainly attributed to strong hydrogen bonds.
These results indicate that SKPE hydrolysates generated by flavourzyme are potential sources of bioactive peptides that could be used in the health food industry. © 2021 Society of Chemical Industry.
最近的研究表明，鱼副产品可用作功能性食品的生物活性肽的来源。鲟鱼皮含有丰富的蛋白质，但在鲟鱼加工过程中通常被丢弃。本工作的目的是从鲟鱼皮蛋白水解物中鉴定和表征生物活性肽。
风味蛋白酶水解 60min 的鲟鱼皮蛋白提取物（SKPE）具有较高的抗氧化活性、二肽基肽酶 IV（DPP-IV）和血管紧张素转化酶（ACE）抑制活性。采用高效液相色谱-串联质谱法鉴定风味酶水解产物中的肽序列。Gly-Asp-Arg-Gly-Glu-Ser-Gly-Pro-Ala（P1）具有最高的 DPPH 自由基清除活性（DPPH IC = 1.93mmol/L）。Gly-Pro-Ala-Gly-Glu-Arg-Gly-Glu-Gly-Gly-Pro-Arg（P11）（DPP-IV IC = 2.14mmol/L）和 Ser-Pro-Gly-Pro-Asp-Gly-Lys-Thr-Gly-Pro-Arg（P12）（DPP-IV IC = 2.61mmol/L）具有最强的 DPP-IV 抑制活性。Gly-Pro-Pro-Gly-Ala-Asp-Gly-Gln-Ala-Gly-Ala-Lys（P6）显示出最高的 ACE 抑制活性（ACE IC = 3.77mmol/L）。分子对接分析表明，P11 和 P12 的 DPP-IV 抑制主要归因于氢键和疏水相互作用，而 P6 的 ACE 抑制主要归因于强氢键。
这些结果表明，风味蛋白酶水解 SKPE 产生的水解物是生物活性肽的潜在来源，可用于保健食品行业。 © 2021 化学工业协会。
