State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.
School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.
J Agric Food Chem. 2021 Sep 29;69(38):11311-11321. doi: 10.1021/acs.jafc.1c03752. Epub 2021 Sep 15.
Abundant conjugated linoleic acid (CLA) producers exist among species. This CLA production is related to the mitigation of LA toxicity. However, there is still a lack of information on the metabolic response underlying this detoxification strategy. In this study, six bifidobacteria strains belonging to three different species were used to characterize growth and CLA accumulation in the presence of LA. A combination of non-targeted metabolomics techniques and biochemical indicators were used to explore metabolic profile changes in response to LA and the expression of important factors driving CLA production in species. The results suggested that free LA had growth inhibitory effects on bifidobacteria, resulting in a global metabolic stress response that caused metabolic reprogramming on all tested strains and promoted malondialdehyde production, inducing a redox imbalance. In particular, a strong decrease in reduced glutathione level was observed in CCFM683 [log(FC) = -3.29]. Furthermore, LA-induced oxidative stress is an important factor driving high CLA production in certain strains.
大量共轭亚油酸（CLA）产生菌存在于 个物种中。这种 CLA 的产生与 LA 毒性的减轻有关。然而，对于这种解毒策略的代谢反应，仍然缺乏信息。在这项研究中，使用了属于三个不同种的六种双歧杆菌菌株来表征在 LA 存在下的生长和 CLA 积累。采用非靶向代谢组学技术和生化指标相结合的方法，探讨了对 LA 的代谢谱变化和驱动 CLA 产生的重要因素在 个种中的表达。结果表明，游离 LA 对双歧杆菌具有生长抑制作用，导致全局代谢应激反应，使所有测试菌株的代谢重编程，并促进丙二醛的产生，诱导氧化还原失衡。特别是，在 CCFM683 中观察到还原型谷胱甘肽水平的强烈下降[log(FC)=-3.29]。此外，LA 诱导的氧化应激是驱动某些菌株高产 CLA 的一个重要因素。
