Hou Jia, Zhang Hao, Ding Jin-Li, Feng Ming-Guang, Ying Sheng-Hua
Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
iScience. 2023 Apr 1;26(4):106551. doi: 10.1016/j.isci.2023.106551. eCollection 2023 Apr 21.
In integrated pest management program (IPM), the compatibility of mycoinsecticides with bioactive fungicides [e.g., unsaturated fatty acids (UFAs)] has attracted more and more attention; however, the mechanisms underlying fungal resistance to UFAs remain largely unknown. In this study, , an entomopathogenic fungus, was used to explore fungal responses to linoleic acid (LA). Genome-wide expression revealed the transcriptomic responses of fungal cells to LA in a stress-intensity-dependent manner. Enrichment analyses indicated that the up-regulated differentially expressed genes (DEGs) are associated with the metabolism of lipid and fatty acids. Notably, a lipid-droplet protein (BbLar1) maintains the intracellular homeostasis of fatty acids and is crucial to fungal tolerance to LA stress, which significantly contributes to fungal compatibility with UFAs. Additionally, BbLar1 links the lipid droplets to global expression profiles in under LA stress. Our investigations provide an initial framework for improving the efficacy of insect pathogenic fungi in practical application.
在害虫综合治理计划（IPM）中，杀真菌昆虫剂与生物活性杀菌剂[如不饱和脂肪酸（UFAs）]的兼容性越来越受到关注；然而，真菌对UFAs的抗性机制在很大程度上仍不清楚。在本研究中，使用一种昆虫病原真菌来探索真菌对亚油酸（LA）的反应。全基因组表达揭示了真菌细胞对LA的转录组反应呈应激强度依赖性。富集分析表明，上调的差异表达基因（DEGs）与脂质和脂肪酸的代谢有关。值得注意的是，一种脂滴蛋白（BbLar1）维持细胞内脂肪酸的稳态，对真菌耐受LA应激至关重要，这显著促进了真菌与UFAs的兼容性。此外，BbLar1在LA应激下将脂滴与球孢白僵菌的整体表达谱联系起来。我们的研究为提高昆虫病原真菌在实际应用中的功效提供了一个初步框架。
