一种使用双水相分配系统分离环状DNA的快速方法。
A rapid method for the isolation of circular DNA using an aqueous two-phase partition system.
作者信息
Ohlsson R, Hentschel C C, Williams J G
出版信息
Nucleic Acids Res. 1978 Feb;5(2):583-90. doi: 10.1093/nar/5.2.583.
A method of isolating circular plasmid DNA from cleared lysates of E. coli is described. Purification is achieved by virtue of the rapid re-annealing kinetics or supercoiled DNA. After a brief denaturation step, double stranded plasmid DNA is separated from denatured chromosomal DNA and RNA in a two-phase partition system using dextran and polyethylene glycol. The method is much more rapid than the conventional dye-centrifugation technique and plasmid DNA of comparable purity and yield is obtained.
描述了一种从大肠杆菌的澄清裂解物中分离环状质粒DNA的方法。纯化是借助超螺旋DNA快速复性动力学实现的。经过短暂的变性步骤后，在使用葡聚糖和聚乙二醇的两相分配系统中，双链质粒DNA与变性的染色体DNA和RNA分离。该方法比传统的染料离心技术快得多，并且可获得纯度和产量相当的质粒DNA。
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