通过双水相体系快速高效纯化DNA自组装结构
Rapid and High-Yielding Purification of DNA Self-Assembled Structures by Aqueous Two-Phase System.
作者信息
Masukawa Marcos K, Takinoue Masahiro
机构信息
Life-Like Materials and Systems, Department of Chemistry, University of Mainz, Mainz, Germany.
Department of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan.
出版信息
Methods Mol Biol. 2025;2901:13-25. doi: 10.1007/978-1-0716-4394-5_2.
Aqueous two-phase systems (ATPS) of dextran and polyethylene glycol (PEG) enable the purification of DNA structures such as DNA origami and DNA nanotubes in times as short as 10 min. This method, which has recovery yields >90% for a typical DNA origami, owes its efficiency to the highly selective partition of the DNA structures in the dextran phase of these emulsions. This purification method is carried out in conditions that promote the structural stability of these structures, making it particularly suitable for DNA nanotechnology. In this protocol, we will describe the materials and methods for purifying DNA origami and quantifying the purification yield by agarose electrophoresis and image analysis.
葡聚糖和聚乙二醇（PEG）组成的双水相系统（ATPS）能够在短短10分钟内实现对DNA结构（如DNA折纸和DNA纳米管）的纯化。这种方法对于典型的DNA折纸回收率>90%，其高效性源于这些乳液葡聚糖相中DNA结构的高度选择性分配。该纯化方法是在促进这些结构的结构稳定性的条件下进行的，使其特别适用于DNA纳米技术。在本方案中，我们将描述通过琼脂糖电泳和图像分析纯化DNA折纸并量化纯化产率的材料和方法。
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