Ma Xiaolan, Zhang Benyin, Xiang Xin, Li Wenjing, Li Jiao, Li Yang, Tran Lam-Son Phan, Yin Hengxia
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China.
College of Eco-Environmental Engineering, Qinghai University, Xining 810016, China.
Plants (Basel). 2024 Oct 13;13(20):2864. doi: 10.3390/plants13202864.
Drought represents a major environmental threat to global agricultural productivity. Employing plant growth-promoting rhizobacteria (PGPR) offers a promising strategy to enhance plant growth and resilience under drought stress. In this study, the strain G124, isolated from the arid region of Qinghai, was characterized at the molecular level, and its ability to enhance plant drought tolerance was validated through pot experiments. The findings revealed that the strain G124 belongs to , with a 99.93% sequence similarity with EB422 and clustered within the same clade. Further analysis indicated that the strain G124 demonstrated a variety of growth-promoting characteristics, including siderophore production, phosphate solubilization, and the synthesis of indole-3-acetic acid (IAA), among others. Moreover, inoculation with G124 resulted in significant enhancements in plant height, leaf area, chlorophyll content, relative water content, and root development in both and seedlings under drought conditions. Additionally, G124 boosted antioxidant enzyme activities and osmolyte accumulation, while reducing malondialdehyde (MDA) and reactive oxygen species (ROS) levels in seedlings exposed to drought. These findings suggest that G124 holds significant promise for enhancing plant drought tolerance and could be effectively utilized in crop management strategies under arid conditions.
干旱是对全球农业生产力的主要环境威胁。利用植物促生根际细菌（PGPR）为增强干旱胁迫下植物的生长和恢复力提供了一种有前景的策略。在本研究中，从青海干旱地区分离出的菌株G124在分子水平上进行了表征，并通过盆栽试验验证了其增强植物耐旱性的能力。研究结果表明，菌株G124属于 ，与EB422的序列相似性为99.93%，并聚集在同一进化枝内。进一步分析表明，菌株G124表现出多种促生长特性，包括铁载体产生、磷溶解以及吲哚-3-乙酸（IAA）的合成等。此外，在干旱条件下，接种G124使 和 幼苗的株高、叶面积、叶绿素含量、相对含水量和根系发育均显著提高。此外，G124提高了抗氧化酶活性和渗透调节物质积累，同时降低了干旱处理的 幼苗中丙二醛（MDA）和活性氧（ROS）水平。这些结果表明，G124在增强植物耐旱性方面具有巨大潜力，可有效应用于干旱条件下的作物管理策略。
