Diagnostica Stago, Asnieres-sur-Seine, France.
Technoclone Herstellung von Diagnostika und Arzneimitteln GmbH, Vienna, Austria.
J Thromb Haemost. 2021 Dec;19(12):2907-2917. doi: 10.1111/jth.15529. Epub 2021 Sep 26.
Thrombin is the pivotal enzyme in the biochemistry of secondary hemostasis crucial to maintaining homeostasis of hemostasis. In contrast to routine coagulation tests (PT or aPTT) or procoagulant or anticoagulant factor assays (e.g. fibrinogen, factor VIII, antithrombin or protein C), the thrombin generation assay (TGA), also named thrombin generation test (TGT) is a so-called "global assay" that provides a picture of the hemostasis balance though a continuous and simultaneous measurement of thrombin formation and inhibition. First described in the early 1950s, as a manual assay, efforts have been made in order to standardize and automate the assay to offer researchers, clinical laboratories and the pharmaceutical industry a versatile tool covering a wide range of clinical and non-clinical applications. This review describes technical options offered to properly run TGA, including a review of preanalytical and analytical items, performance, interpretation, and applications in physiology research and pharmacy.
凝血酶是二次止血生物化学中的关键酶，对维持止血的体内平衡至关重要。与常规凝血试验（PT 或 aPTT）或促凝血或抗凝因子测定（如纤维蛋白原、VIII 因子、抗凝血酶或蛋白 C）不同，凝血酶生成试验（TGA），也称为凝血酶生成试验（TGT），是一种所谓的“整体测定法”，通过连续和同时测量凝血酶的形成和抑制，提供止血平衡的图像。该测定法于 20 世纪 50 年代初首次被描述为一种手动测定法，为了使该测定法标准化和自动化，研究人员、临床实验室和制药行业都在努力提供一种多功能工具，涵盖广泛的临床和非临床应用。这篇综述描述了正确运行 TGA 所提供的技术选择，包括对分析前和分析项目、性能、解释以及在生理学研究和药学中的应用的综述。
