Zhang Shijuan, Wei Yuhong, Liu Jinxia, Zhuang Yutian
Department of Critical Care Medicine, Yidu Central Hospital of Weifang, Weifang, Shandong, 262500, People's Republic of China.
Department of Gastroenterology First Ward, Yidu Central Hospital of Weifang, Weifang, Shandong, 262500, People's Republic of China.
J Inflamm Res. 2021 Sep 9;14:4567-4574. doi: 10.2147/JIR.S316169. eCollection 2021.
Sepsis is a heterogeneous syndrome with a life-long threat caused by infection. This study aimed to investigate the clinical function of miR-940 and its influence on cardiomyocyte models.
The relative expression of miR-940 was assessed by qRT-PCR and the roles in the clinical diagnosis of miR-940 were revealed by the ROC curve. The relationship between miR-940 and clinical parameters was validated by Pearson analysis. The sepsis rat models were established by treatment with cecal ligation and perforation (CLP) and clinical items including left ventricular systolic pressure (LVSP), left ventricular and end-diastolic pressure (LVEDP), maximum rate of increase/decrease in left ventricular blood pressure (± dp/dt) as well as troponin (cTnl), creatine kinase isoenzyme (CK-MB), TNF-α, IL-1β, and IL-6 were detected.
The finding of qRT-PCR accentuated that the relative expression of miR-940 was significantly decreased in sepsis patients and CLP-stimulated models. The ROC curve proposed that miR-940 could be a satisfactory diagnostic biomarker for sepsis patients. Pearson analysis reinforced the expression of miR-940 was negatively associated with the PCT, WBC, CRP, Scr, SOFA score, and APACHE II score. The outcome of CLP-steered rat verified that overexpression of miR-940 inhibited the detrimental effects of CLP on myocardial dysfunction and inflammation reactions.
The downregulation of miR-940 was reported and it might be an underlying diagnostic marker in sepsis patients. Overexpression of miR-940 protected myocardial function from damage and inflammation induced by CLP.
脓毒症是一种由感染引起的具有终身威胁的异质性综合征。本研究旨在探讨miR-940的临床功能及其对心肌细胞模型的影响。
采用qRT-PCR评估miR-940的相对表达，并通过ROC曲线揭示miR-940在临床诊断中的作用。通过Pearson分析验证miR-940与临床参数之间的关系。采用盲肠结扎穿孔术（CLP）建立脓毒症大鼠模型，并检测包括左心室收缩压（LVSP）、左心室舒张末期压力（LVEDP）、左心室血压最大上升/下降速率（±dp/dt）以及肌钙蛋白（cTnl）、肌酸激酶同工酶（CK-MB）、TNF-α、IL-1β和IL-6等临床指标。
qRT-PCR结果显示，脓毒症患者和CLP刺激模型中miR-940的相对表达显著降低。ROC曲线表明，miR-940可能是脓毒症患者理想的诊断生物标志物。Pearson分析进一步证实，miR-940的表达与PCT、WBC、CRP、Scr、SOFA评分和APACHE II评分呈负相关。CLP诱导的大鼠实验结果证实，miR-940过表达可抑制CLP对心肌功能障碍和炎症反应的有害影响。
本研究报道了miR-940的下调，它可能是脓毒症患者潜在的诊断标志物。miR-940过表达可保护心肌功能免受CLP诱导的损伤和炎症影响。
