Arslan Burak, Arslan Gökçe Ayhan, Tuncer Aslı, Karabudak Rana, Dinçel Aylin Sepici
Department of Medical Biochemistry, Gazi University Faculty of Medicine, Ankara, Turkey.
Department of Medical Biochemistry, Erciş State Hospital, Van, Turkey.
Front Neurol. 2021 Aug 30;12:716195. doi: 10.3389/fneur.2021.716195. eCollection 2021.
The aim of this pilot study was to evaluate dynamic thiol-disulfide homeostasis as a novel oxidative stress parameter in multiple sclerosis (MS), neuromyelitis optica spectrum disorders (NMOSD), and myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) to better understand the role of thiol homeostasis in neuroimmunological diseases. A total of 85 participants were included in this study, consisting of 18 healthy controls, 52 patients diagnosed with MS, seven with NMOSD, and eight with MOGAD. We measured total thiol (-SH+-S-S-) and native thiol (-SH) levels in the serum of all the participants, and in a subset of patients ( = 11), these parameters were investigated in paired cerebrospinal fluid (CSF) and serum samples. Dynamic disulfide concentrations were calculated separately. Finally, we determined if there was any relationship between clinical features and dynamic thiol homeostasis. There was a statistically significant difference between serum and CSF levels of biomarkers of thiol homeostasis. Serum total thiol (317.88 ± 66.04) and native thiol (211.61 ± 44.15) levels were significantly lower in relapsed patients compared to those in remission (368.84 ± 150.36 vs. 222.52 ± 70.59, respectively). Oxidative stress plays a crucial role in the physiopathology of neuroimmunological diseases. Thiol homeostasis may be useful for monitoring disease activity.
这项初步研究的目的是评估动态硫醇-二硫键稳态作为多发性硬化症（MS）、视神经脊髓炎谱系障碍（NMOSD）和髓鞘少突胶质细胞糖蛋白抗体相关疾病（MOGAD）中一种新的氧化应激参数，以更好地理解硫醇稳态在神经免疫疾病中的作用。本研究共纳入85名参与者，包括18名健康对照者、52名诊断为MS的患者、7名NMOSD患者和8名MOGAD患者。我们测量了所有参与者血清中的总硫醇（-SH+-S-S-）和天然硫醇（-SH）水平，并且在一部分患者（n = 11）中，对配对的脑脊液（CSF）和血清样本中的这些参数进行了研究。分别计算动态二硫键浓度。最后，我们确定临床特征与动态硫醇稳态之间是否存在任何关系。硫醇稳态生物标志物的血清和脑脊液水平之间存在统计学上的显著差异。复发患者的血清总硫醇（317.88±66.04）和天然硫醇（211.61±44.15）水平显著低于缓解期患者（分别为368.84±150.36和222.52±70.59）。氧化应激在神经免疫疾病的病理生理过程中起关键作用。硫醇稳态可能有助于监测疾病活动。
