Subbotin Sergei A
California Department of Food and Agriculture, Plant Pest Diagnostics Center, 3294 Meadowview Road, Sacramento, CA, 95832.
J Nematol. 2021 Sep 2;53. doi: 10.21307/jofnem-2021-077. eCollection 2021.
During the winter and spring of 2021, the root-knot nematodes were extracted from samples of galled roots of potted American pitcher plants ( sp.). Samples were collected from a botanical garden nursery in Los Angeles County, California. The root-knot nematode was identified by molecular methods as . In the USA, was initially found in Texas, and subsequently reported from Arkansas and Florida. Molecular characterization of the Californian isolate was done using the analysis of the D2-D3 of 28S rRNA, ITS rRNA, mitochondrial l-rRNA, , and gene sequences. Some rRNA gene clusters of were similar with those of . Possible hybridization events within mitotic parthenogenetic root-knot nematodes are discussed. This study confirmed that reliable diagnostics of should be based on mtDNA sequence analysis. This is a first report of from sp. and California. Consequently, this nematode was considered to be eradicated from this botanical garden nursery and the State of California.
2021年冬春季节，从盆栽美国猪笼草（品种未提及）带虫瘿根的样本中提取根结线虫。样本采自加利福尼亚州洛杉矶县的一个植物园苗圃。通过分子方法将该根结线虫鉴定为南方根结线虫。在美国，南方根结线虫最初在得克萨斯州被发现，随后在阿肯色州和佛罗里达州也有报道。利用28S rRNA、ITS rRNA、线粒体l-rRNA、以及其他基因序列的D2-D3区域分析对加利福尼亚州南方根结线虫分离株进行分子特征分析。南方根结线虫的一些rRNA基因簇与其他线虫的相似。讨论了有丝分裂孤雌生殖根结线虫内可能的杂交事件。本研究证实，南方根结线虫的可靠诊断应基于线粒体DNA序列分析。这是关于南方根结线虫在美国猪笼草品种及加利福尼亚州的首次报道。因此，该线虫被认为已从这个植物园苗圃和加利福尼亚州根除。
