Smt. P. K. Kotecha Mahila Mahavidyalaya, Bhusawal, Dist.-Jalgaon, Maharashtra-425 201, India.
Indofil Industries Limited, Thane, Maharashtra-400 607, India.
Anal Methods. 2021 Sep 16;13(35):3930-3939. doi: 10.1039/d1ay00822f.
A simple derivatization reverse-phase high performance liquid chromatographic method for the simultaneous analysis (separation and quantification) of zineb and hexaconazole has been optimized and validated. The method was carried out on an Agilent Eclipse plus C18 column (150 × 4.6 mm; 5 μm), with a mobile phase comprising acetonitrile + methanol (80 + 20 v/v): water (0.1% v/v trifluoroacetic acid) (60 : 40 v/v) at a flow rate of 1.0 mL minute, and the quantification was achieved at 272 nm and 205 nm. The retention times of zineb and hexaconazole were found to be 6.4 min and 7.6 min, respectively. The performance of the method was validated according to the SANCO, CIPAC and ICH guidelines for specificity, selectivity, linearity, precision, accuracy, limit of detection, limit of quantification and robustness. The developed method was successfully applied for the estimation of zineb and hexaconazole in a pesticide dosage form.
已对一种简单的衍生化反相高效液相色谱法进行了优化和验证，用于同时分析（分离和定量）代森锰锌和己唑醇。该方法在安捷伦 Eclipse plus C18 柱（150×4.6mm；5μm）上进行，流动相由乙腈+甲醇（80+20v/v）：水（0.1%v/v 三氟乙酸）（60：40v/v）组成，流速为 1.0mL/min，在 272nm 和 205nm 处进行定量。代森锰锌和己唑醇的保留时间分别为 6.4min 和 7.6min。该方法的性能根据 SANCO、CIPAC 和 ICH 指南进行了专属性、选择性、线性、精密度、准确度、检测限、定量限和稳健性的验证。所开发的方法成功应用于农药制剂中代森锰锌和己唑醇的测定。
