D. D. N. Bhole College, Bhusawal, Dist.-Jalgaon, Maharashtra-425201, India.
Jain Irrigation Systems Limited, Jalgaon, Maharashtra-425001, India.
Anal Methods. 2022 Mar 3;14(9):907-920. doi: 10.1039/d1ay01926k.
A novel, rapid and simple reverse-phase high performance liquid chromatography (RP-HPLC) method for the simultaneous determination of three pesticides - mancozeb, azoxystrobin and difenoconazole by derivatization with ethyl iodide is presented. Analysis was performed on a C18 column (Agilent Eclipse plus, 150 mm × 4.6 mm; 5 μ) with the mobile phase consisting of acetonitrile + methanol (90 + 10 v/v) - water (0.1% v/v trifluoroacetic acid) (60 : 40, v/v) pumped isocratically at a flow rate of 1.0 mL min and detection wavelength of 205 nm and 272 nm. The factors affecting the derivatization reaction and separation conditions were carefully evaluated and optimized. The method was linear over the concentration range of 3.50 mg L to 31.48 mg L for mancozeb, 0.32 mg L to 2.85 mg L for azoxystrobin and 0.32 mg L to 2.89 mg L for difenoconazole. The new method was successfully applied for the analysis of mancozeb, azoxystrobin and difenoconazole in the pesticide formulation with range recoveries of 99.46% to 100.76%, 99.07% to 101.09% and 98.59% to 101.59%, respectively. The present method is suitable and favorable for the simultaneous separation and analysis of tertiary mixture analytes on account of its sensitivity, rapidity and cost-effectiveness. In addition, it could have excellent application prospects for the simultaneous determination of all three pesticides in other formulated products.
本文提出了一种新颖、快速、简单的反相高效液相色谱（RP-HPLC）方法，通过乙基碘化物衍生化，可同时测定三种农药 - 代森锰锌、唑菌酯和苯醚甲环唑。分析在 C18 柱（Agilent Eclipse plus，150mm×4.6mm；5μm）上进行，流动相由乙腈+甲醇（90+10v/v）-水（0.1%v/v 三氟乙酸）（60:40，v/v）等度洗脱，流速为 1.0mL min，检测波长为 205nm 和 272nm。仔细评估和优化了影响衍生化反应和分离条件的因素。该方法在代森锰锌的浓度范围为 3.50mg L 至 31.48mg L、唑菌酯的浓度范围为 0.32mg L 至 2.85mg L、苯醚甲环唑的浓度范围为 0.32mg L 至 2.89mg L 时呈线性。该新方法成功应用于农药制剂中代森锰锌、唑菌酯和苯醚甲环唑的分析，回收率范围分别为 99.46%至 100.76%、99.07%至 101.09%和 98.59%至 101.59%。该方法灵敏度高、快速、经济，适用于三元混合物分析物的同时分离和分析。此外，它可能在同时测定其他制剂产品中所有三种农药方面具有极好的应用前景。
