School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, 255049, China.
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, 255049, China.
Biosens Bioelectron. 2021 Dec 15;194:113601. doi: 10.1016/j.bios.2021.113601. Epub 2021 Sep 6.
In this work, a novel electrochemiluminescence (ECL) aptasensor was structured for the detection of four organophosphorus pesticides (OPs). Firstly, multi-walled carbon nanotubes (MWCNTs) were used to create a favorable loading interface for the fixation of tris (2, 2'-bipyridyl) ruthenium (II) (Ru (bpy)). At the same time, copper (core)-gold (shell) bimetallic nanoparticles (Cu@Au NPs) were synthesized in the aqueous phase for the sensor construction. Gold nanoparticles (Au NPs) could promote the electrochemiluminescence intensity of Ru (bpy) with high efficiency by catalyzing the oxidation process of tri-n-propylamine (TPrA). Compared with the Au NPs, Cu@Au NPs increased the solid loading of Au NPs by virtue of the large specific surface area of copper nanoparticles (Cu NPs), which could further improve the sensitivity of aptasensor. When OPs were added, the ECL intensity was significantly reduced, and the concentration of OPs could be detected through the ECL intensity. Under the optimum conditions, the aptasensor had a wider dynamic range and ultra-low detection limit for the detection of four pesticides: profenofos, isocarbophos, phorate, and omethoate, and their detection limits were 3 × 10 ng/mL, 3 × 10 ng/mL, 3 × 10 ng/mL, and 3 × 10 ng/mL respectively (S/N = 3). The aptasensor had the merits of good stability, reproducibility, and specificity, and had a favorable recovery rate in detecting OPs residues in vegetables. This work provided an effective method for the construction of a simple, rapid, and sensitive biosensor.
在这项工作中，构建了一种新颖的电化学发光（ECL）适体传感器，用于检测四种有机磷农药（OPs）。首先，多壁碳纳米管（MWCNTs）用于为三（2,2'-联吡啶）钌（II）（Ru（bpy））的固定创建有利的负载界面。同时，在水相中合成了铜（核）-金（壳）双金属纳米粒子（Cu@Au NPs）用于传感器构建。金纳米粒子（Au NPs）通过催化三丙胺（TPrA）的氧化过程，以高效率增强 Ru（bpy）的电化学发光强度。与 Au NPs 相比，Cu@Au NPs 通过铜纳米粒子（Cu NPs）的大比表面积增加了 Au NPs 的固载量，从而进一步提高了适体传感器的灵敏度。当加入 OPs 时，ECL 强度显着降低，并且可以通过 ECL 强度来检测 OPs 的浓度。在最佳条件下，该适体传感器对四种农药：丙溴磷、异稻瘟净、甲拌磷和氧乐果的检测具有更宽的动态范围和超低检测限，其检测限分别为 3×10ng/mL、3×10ng/mL、3×10ng/mL 和 3×10ng/mL（S/N=3）。该适体传感器具有良好的稳定性、重现性和特异性的优点，并且在检测蔬菜中 OPs 残留时具有良好的回收率。这项工作为构建简单、快速、灵敏的生物传感器提供了一种有效的方法。
